Thermo Fisher Scientific
ArC™ Amine Reactive Compensation Bead Kit
A10346
Info: Supplier Page
My research area includes immune phenotyping of primary or metastatic tumors. I often do a complex flow cytometry panel for my analysis. The ArC™ Amine Reactive Compensation Bead is very helpful in doing compensation or unmixing.
Compensation Control for Flow Cytometry.
Fixable viability dye.
After staining the beads with the viability dye, subsequent wash with 1x PBS will significantly improve the staining index.
The ArC Amine Reactive Beads are ready-to-use polystyrene-based beads. The results here are shown for a single color compensation control prepared for Zombie UV-fixable dye. The beads are a mixture of reactive and non-reactive beads and give a very good positive and negative signal required for compensation.1 drop of positive beads is first stained with Zombie UV dye (at 1:200 dilution) for 20 mins on the ice and then washed with 1x PBS. After washing negative beads are mixed with stained positive beads and run on BD Symphony A3 for single-color controls.
N/A
Very easy to prepare the single color control.
Expensive.
I would definitely use it as it saves a lot of time.