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Brilliant Violet 510™ anti-human CD69 Antibody
310935
FN10
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To test this antibody, I stained PBMCs for 30 minutes at room temperature in the dark at serial dilutions of 1:25, 1:50, 1:100, 1:200, and 1:400.
Flow cytometry
Human PBMC
30 minutes at room temperature.
PBS/BSA
None
v-525/50
As you can see from the stainings CD69 is not well expressed in PBMC, and would have been better to test in tissue cells. Nevertheless I would go ahead and stain at a 1:100 dilution.
N/A
Easy to use.
Not bright in blood, but it was expected.
I'd use it again for sure!