LipidSpot 610 From Biotium

University of Texas Southwestern Medical Center
Surgery
Research Scientist

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Company:

Biotium

Product Name:

LipidSpot 610

Catalog Number:

70069

I used this to stain lipids in one of my adipogenic induction experiments using human mammary pre-adipocytes. After treatment with adipogenic factors, the cells clearly showed an accumulation of lipid droplets under a bright field and were positively stained with LipidSpot 610. This was confirmed using the TexasRed channel under fluorescence microscopy.

Experimental Design and Results Summary

Application

Immunofluorescence

Starting Material

Preadipocytes BR-F

Tips

1:1000 on live cells

Results Summary

It stained lipid droplets specifically and the staining merged perfectly with grape-like lipid structures under the bright field.

DOI or PMID #

N/A

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Summary

The Good

Can be directly used on live cells without fixation, it is also cheap but of good quality.

The Bad

N/A

The Bottom Line

Showed reliable and specific stain of lipid.

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