ThermoFisher Scientific
Tetramethylrhodamine, Methyl Ester, Perchlorate (TMRM)
T668
Info: Supplier Page
TMRM is a cell-permeant, cationic, red fluorescent dye that is readily sequestered by active mitochondria and can be detected by either microscopy or flow cytometry. If the mitochondria membrane potential is perturbed the fluorescence intensity of TMRM decreases.
Flow cytometry
Neuro2a cells
Optimize the concentration of TMRM according to cell type.
TMRM dye was used to confirm loss of mitochondria membrane potential due to CCCP. Mitotracker Green that stains mitochondria irrespective of membrane potential was used as a control. Fluorescent cells were analysed using flow cytometry.
N/A
Simple and fast staining, low concentrations required, and stable in solution.
None.
Good reagent.