Highly Recommended Genomic DNA Isolation Kit From New England BioLabs

The Scripps Research Institute
Immunology Microbiology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

New England Biolabs

Product Name:

Monarch® Genomic DNA Purification Kit

Catalog Number:

T3010S

In order to clone bacteria genes, I must extract genomic DNA (gDNA) using this kit, which is more efficient than the traditional cetyltrimethylammonium bromide (CTAB) method.

Experimental Design and Results Summary

Application

Genomic DNA isolation

Starting Material

Overnight grown bacterial culture

Tips

1 milliliter of over grown culture is sufficient. Do not take more culture for the prep.

Results Summary

I isolated many gDNAs from my gene knockout bacterial library and verified the strains by PCR and DNA sequencing. This could be possible only because of pure yields.

DOI or PMID #

N/A

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Summary

The Good

User-friendly protocols and suitable for different tissue samples.

The Bad

N/A

The Bottom Line

Highly recommended kit for fast and high quality genomic DNA isolation.

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