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Date Published
ELISpot feasibility
(1)
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June 26, 2015
Overall the product has proven to be effective. Although, the description lacks the identification of required reagents that must be ...
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Confocal Microscope
(1)
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Leica Microsystems
June 26, 2015
My project involves elucidating the roles of Eps15 Homology Domain containing protein 4 (EHD4) in the kidney. EHD4 is a member of the EHD ...
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MQ10 Flow Cytometer
(1)
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Miltenyi Biotec
June 26, 2015
We are examining the role of innate and adaptive immune cells in disease states in large population studies. We needed a machine capable of...
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Benchtop PCR plate centrifuge from Labnet
(1)
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Corning Life Sciences
June 25, 2015
This is a convenient and compact benchtop centrifuge for PCR plates.
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Treg Isolation
(1)
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Miltenyi Biotec
June 25, 2015
This Kit is very useful for isolating CD4+CD25+ Tregs from LN and Spleen, in order to culture Tregs, using them for RT PCR or for adoptive ...
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Use of Isotemp 205 waterbath
(1)
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Fisher Scientific
June 25, 2015
This Fisher Scientific Isotemp 205 waterbath was used in the heat inactivation of FBS.
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BACH2 polyclonal antibody for IHC
(1)
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June 25, 2015
BACH2 is known to play a role in B cell maturation. I have used the BACH2 antibody to assess BACH2 protein expression in high grade B-cell ...
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GAPDH Western Blot detection
(1)
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Cell Signaling Technology
June 25, 2015
Our labs longstanding goal is to understand the mistranslocation of cancer specific proteins. Here in this study we are evaluating different...
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Clean and specific PIAS1 antibody
(1)
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Cell Signaling Technology
June 24, 2015
PIAS1, an E3-type SUMO ligase tethering SUMO moiety to substrate proteins, is crucial for protein functions and transcriptional coregulation...
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Bio Rad SYBR Green Supermix
(1)
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Bio-Rad
June 24, 2015
For these experiments we were assessing Aryl hydrocarbon Receptor activation in bone marrow derived dendritic cells. Precursor cells were ...
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Classic reagent for RNA/DNA/protein isolation from cells and tissues
(1)
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June 24, 2015
We use TRIzol to extract RNA from various cells and tissues for PCR amplification. The general aim is to study changes in gene expression. ...
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Rabbit anti-V5 immunofluorescence microscopy
(1)
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Cell Signaling Technology
June 24, 2015
This antibody was used to perform immunofluorescence microscopy on HeLa cells transfected with a V5 containing construct. Several anti-V5 ...
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Use of Rad Source irradiator in the irradiation of splenocytes
(1)
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June 24, 2015
The Rad Source RS-2000 Biological System is used in the irradiation of a single cell suspension of splenocytes.
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Used for H&E staining
(1)
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Fisher BioReagents
June 24, 2015
This is a very good kit for H&E staining, the nuclear staining is strong enough to separated and the color is very nice, especially for the ...
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Good Miniprep Kit
(1)
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June 23, 2015
I use this kit when I need to make relatively small amounts of DNA to use for restriction digestion, colony PCR, etc. I like this kit ...
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Good kit for transfecting cells
(1)
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June 23, 2015
I use this kit to transfect HEK293T cells in the lab when making virus. I like this kit because I can achieve 90+% transfection with a ...
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RNeasy Plus Mini Kit (250)
(1)
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QIAGEN - Sample to Insight
June 23, 2015
For these experiments we were assessing Aryl hydrocarbon receptor activation in bone marrow derived dendritic cells. Precursor cells were ...
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Robust and Efficient Heating Block for traditional PCR reactions
(1)
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Bio-Rad
June 23, 2015
In the age of digital and real-time PCR, traditional PCR may have been cornered to the backseat. However, for isolation of large amounts of ...
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Total caspase 3 detection
(1)
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Cell Signaling Technology
June 23, 2015
Our lab's longstanding goal is to unravel new protein biomarkers in cancer and elucidate their functional role in tumorigenesis. Here in ...
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Good plasmid isolation kit
(1)
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Omega Bio-Tek, Inc.
June 22, 2015
I use this kit when I need to isolate plasmid DNA in the lab for cloning purposes. I like this kit because I never have trouble using the ...
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Easy and high titer production of retroviruses from a producer cell line
(1)
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June 22, 2015
Generation of retroviral vectors from a producer cell needs to be reliable for downstream applications for therapeutics development. We used...
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Plasmid maxiprep kit especially good for in vivo experiments
(1)
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June 22, 2015
The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit could be used to extract plasmid in maxi in a short amount of time, it just takes about ...
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Valuable Isotype control for flow cytometry of peritoneal macrophages to confirm F4/80 staining
(1)
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June 22, 2015
Peritoneal macrophages can be easily sorted and analyzed via flow cytometry using the pan-macrophage marker - F4/80. However, to confirm and...
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Good RAF1 antibody for Western Blot
(1)
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MilliporeSigma
June 22, 2015
I was looking at RAF1 expression in iPSCs to study RAF1 function in human iPSC-derived cardiomyocytes. I used this product to detect ...
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Good quality L-broth with low price
(1)
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USBiological
June 19, 2015
We use this particular L-Broth (Luria Broth) because the LB powder is same quality as other companys' products, but the price has a huge ...
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Total ROS detection in eosinophils
(1)
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Enzo Life Sciences, Inc.
June 19, 2015
I work on trying to determine the source of ROS production in eosinophils following the engagement of a eosinophil specific surface receptor...
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Quick and Easy Mix for quantifying multiple samples for retroviral titer
(1)
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June 19, 2015
Retroviruses are critical in most therapeutic and gene transduction experiments. To optimize their performance, quantifying their titer post...
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Analysis of T-cell Central Memory Population
(1)
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BioLegend
June 19, 2015
A small fraction of Naive T cells upon primary exposure to foreign or self antigens differentiate to form a population called T central ...
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Fast Plasmid Midiprep Kit
(1)
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June 19, 2015
We use this kit as it is very convenient for doing midi-preparation of plasmid from bacterial culture, the whole process takes about 30 ...
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Easy kit for plasmid midipreps
(1)
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Omega Bio-Tek, Inc.
June 18, 2015
I perform a lot of cloning in the lab and use this kit to isolate plasmid DNA from bacteria. I like using this kit because it is pretty easy...
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Fast and easy but do not let the warranty expire
(1)
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Bio-Rad
June 18, 2015
The trans-blot turbo system is easy and fast to use especially if combined with the Bio-rad transfer kit that combines all the solution, ...
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Anti-Canine CD25 PE
(1)
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June 18, 2015
CD25 was used as a marker to identify CD4+FoxP3+ regulatory T cells in dogs with cancer. A significant increased number of Tregs was found ...
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Anti-6X His tag antibody
(1)
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Abcam
June 18, 2015
MCF7 cells were grown in DMEM medium supplemented with 10% FBS, 100µg/ml streptomycin and 100µg/ml penicillin in 5% CO2 at 37°C. Cells were ...
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Easy-to-use Retroviral Titer kit
(1)
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June 18, 2015
Retroviral quantification is critical especially when combined with other biomolecular assays for precise control of signaling mechanisms. ...
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Efficient Reverse Transcription via Taqman Reagents leads to precise quantification of retroviral titer
(1)
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June 17, 2015
Quantification of retroviral titer can be tricky considering that they contain RNA as the genetic construct rather than DNA. Conversion of ...
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The best LAP antibody for humans that I have found
(1)
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BioLegend
June 17, 2015
This antibody detects membrane bound latent TGF-b1 by staining of the latency associated peptide (LAP). I use only 5ul instead of the ...
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Normicn in mammalian cell culture media to prevent microbial contamination
(1)
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June 17, 2015
We are performing compound toxicity and signalling studies on diverse neuronal cell lines. Some cells like neuro2A are highly sensitive to ...
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High Fidelity Polymerase Product
(1)
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June 17, 2015
I chose this product because PrimeSTAR GXL DNA Polymerase is a high fidelity polymerase for PCR amplification, it can be used for GC-rich ...
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Stable and efficient reducing of liposomal size
(1)
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Avanti Polar Lipids Inc.
June 17, 2015
Development of liposomal formulations for gene therapy and drug delivery is a common technique used in most transfection protocols. In order...
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Convenient but not made very well
(1)
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June 16, 2015
As with any lab, we do quite a bit of centrifuging. We purchased this Mini Centrifuge because of its convenience and its ability to fit a ...
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Good anti-mouse CD11b antibody
(1)
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Thermo Fisher Scientific
June 16, 2015
The objective of this experiment was to identify the percentage of CD11b positive cells in the splenocytes isolated from Balb/C mouse. To ...
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pCREB expression in N2A cells
(1)
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Abcam
June 16, 2015
Our work focuses on gene and protein changes (epigenetic modulators) involved in neurotrophic and neuroprotective activity. We used this ...
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A good antibody for platelet identification
(1)
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June 16, 2015
This antibody is generally used to identify platelets in the whole blood. In combination with the forward scatter/side scatter, this ...
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Precise Viral RNA Isolation from supernatant of retroviral producer cell lines
(1)
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QIAGEN - Sample to Insight
June 16, 2015
Producer cell lines to obtain retroviruses and adenoviruses are commonly used in most viral therapeutics and transduction labs. In order to ...
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Very good anti-Mouse CD8 antibody
(1)
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June 15, 2015
The aim of this experiment was to study the T cell subsets in mouse spleen. I tested whether this anti-CD8 PE/Cy7 antibody from BD ...
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pCREB expression in Neuronal cells
(1)
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MilliporeSigma
June 15, 2015
We used this product to check the pCREB expression in neuronal cells and its role in neural functioning. Compared to other company's ...
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Brilliant fluorochrome for a dim marker
(1)
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BioLegend
June 15, 2015
This antibody was used in a staining panel to identify Tregs in the thymus and the spleen. The CD25-Brilliant Violet 421 antibody had a ...
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Precise elemental composition in a timely fashion provided by Atlantic Microlab, Inc
(1)
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June 15, 2015
Our lab works with formulation of various drug salts for testing therapeutic efficiencies and pharmacology. Apart from the host of other ...
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Expression of activation marker CD69 by flow cytometry
(1)
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BioLegend
June 15, 2015
T cells and NK cells upregulate surface marker CD69 upon activation. CD69 is one of the early activation markers in T cell stimulation/ ...
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Nkp46 IFC detection
(1)
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R&D Systems, a Bio-Techne Brand
June 12, 2015
Our lab's longstanding goal is to unravel new protein biomarkers in cancer and elucidate their functional role in tumorigenesis. In this ...
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