Total caspase 3 detection

Cancer bio
MDA
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Caspase-3

Catalog Number:

9665

Info: View Product View Product Specs

Browse Similar Products: Caspase-3 Antibodies

Our lab's longstanding goal is to unravel new protein biomarkers in cancer and elucidate their functional role in tumorigenesis. Here in this study we evaluate the expression of caspase-3 protein in colon cancer cells upon drug treatments using this antibody. From the results it can be seen that this antibody specifically detects caspase 3 protein cells. There was no cleaved caspase-3 detection in these cells. This is a very good antibody for detection of total caspase 3 protein.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Colon cancer cell lines

Primary Incubation

Caspase 3

Blocking Agent

5% BSA

Secondary Incubation

Rabbit secondary

Tertiary Incubation

NA

Detection

Western Blot: Film

Results Summary

From the results it could be seen that only total protein levels of caspase-3 are detectable here. M represents marker.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very sensitive

The Bad

None

The Bottom Line

Good for detecting Total caspase 3

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