pCREB expression in N2A cells

June 16, 2015

chemical biology
indian institute of chemical technology

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Company:

Abcam

Product Name:

Anti-CREB (phospho S133) antibody

Catalog Number:

ab32096

Info: View Product View Product Specs

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Our work focuses on gene and protein changes (epigenetic modulators) involved in neurotrophic and neuroprotective activity. We used this product to check the expression of pCREB in neurotrophicity.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

N2A cells

Primary Incubation

1:250 in 2%BSA, 0vernight, 4c

Blocking Agent

2%BSA in PBST

Secondary Incubation

1:400 Rabbit alexa 488, 2h Room temperature

Tertiary Incubation

No tertiary incubation

Detection

Fluorescence microscope

Results Summary

pCREB expression is changing in neurotrophicity.

Additional Notes

Before blocking, fix the cells in 4% PFA, permibilize the cells with in 0.2% triton x-100 for 15min

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Recommended for Immunocytochemistry

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