Avanti Polar Lipids Inc
Avanti® Mini-Extruder
610000
Development of liposomal formulations for gene therapy and drug delivery is a common technique used in most transfection protocols. In order to develop optimized therapeutics, reduction and optimization of size is critical toward in vivo applications. We used the Avanti Extruder to obtain uniformly sized unimodal liposomal vesicles to encapsulate siRNA, plasmid DNA and retroviral particles for delivery to human cells.
Liposomal Therapeutics
Re-suspended solution of lipids/Multilamellar vesicular solution of liposomes
A liposomal solution was passed 11 times through the extruder with the 100 nm filter membrane in place. Liposomes were sized before and after extrusion
Make sure the extrusion takes place above the highest transition temperature of the lipids being used. Be gentle with the membranes as they can tear easily
As shown in the image, uniformly sized vesicles of 100-200 nm were efficiently obtained within 10 minutes of extrusion. Pre-extrusion, the liposomal sized were between 300-500 nm in diameter.
The setup is manual and fits easily on a lab bench. Membranes and syringes also available from Avanti.
None
Fast, Reliable
Syringes can start leaking over a prolonged use of time. Use teflon tape to tighten gaps.
Small and capable extruder for formulation of liposomal therapeutics.