Avanti Polar Lipids Inc
Avanti® Mini-Extruder
610000
Development of liposomal formulations for gene therapy and drug delivery is a common technique used in most transfection protocols. In order to develop optimized therapeutics, reduction and optimization of size is critical toward in vivo applications. We used the Avanti Extruder to obtain uniformly sized unimodal liposomal vesicles to encapsulate siRNA, plasmid DNA and retroviral particles for delivery to human cells.
Make sure the extrusion takes place above the highest transition temperature of the lipids being used. Be gentle with the membranes as they can tear easily
Fast, Reliable
Syringes can start leaking over a prolonged use of time. Use teflon tape to tighten gaps.
Small and capable extruder for formulation of liposomal therapeutics.