Fast and easy but do not let the warranty expire

NYULMC
institute of systems genetics
post doctoral fellow

Overall

Performance

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

Trans-blot turbo

Catalog Number:

170-4155

The trans-blot turbo system is easy and fast to use especially if combined with the Bio-rad transfer kit that combines the solution, membranes and filters for FAST transfer (10 minutes and it is DONE!). Unfortunately because of the high heat and power generated by the machine, quite delicate contacts (pogo pins positioned on the back of the transfer cassettes), and the nature of the buffer used, the system is very prone to breakage. In our experience after about one year of use the machine needs to be repaired or substituted. So don't let the warranty expire because you'll need it.

Experimental Design and Results Summary

Application

Transfer blot

Starting Material

Protein gel

Protocol Overview

Acrylamide gels (2midi or 1 mini) are positioned in a transfer stack with PVDF-F methanol activated membrane. The run selected and after transfer the membrane is transferred to blocking buffer

Tips

Clean well with distilled water after each use

Results Summary

Very fast and easy transfer of protein gels onto blotting membrane

Features Summary

Easy and fast

Additional Notes

None

Image Gallery

Summary

The Good

Easy and fast

The Bad

Prone to breakage

The Bottom Line

If you are looking for a fast and easy instrument for transfer this is the one. If you want a long lasting machine this is maybe not your best choice

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