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Date Published
Good secondary antibody
(1)
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Thermo Fisher Scientific
April 15, 2015
This is a good secondary antibody that detects primary mouse antibodies and gives strong signal for fluorescent microscopy.
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Cost effective and easy
(1)
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Thermo Fisher Scientific
April 15, 2015
Used this kit to convert to cDNA from primary neurons, cell lines, and DNA from tissues with the ultimate goal of running qPCR on the ...
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Good source of IL-13
(1)
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April 14, 2015
Used this IL-13 to activate JAK/STAT signaling in my cells. IL-13 increased STAT6 phosphorylation, which was my assay for activation of JAK/...
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EPAC1 Western blot
(1)
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Cell Signaling Technology
April 14, 2015
Western blot of extracts of whole mouse brain was done using EPAC1 antibody. We wanted to see whether the protein was expressed in NHBE ...
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Reliable CD45 antibody
(1)
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April 14, 2015
We were trying to distinguish microglia and astrocytes, by flow cytometry, from mixed glial cultures cultured for 4-6 weeks from pup brains....
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Good antibody for detecting Lamin A/C
(1)
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Santa Cruz Biotechnology, Inc.
April 14, 2015
I used this antibody to detect Lamin A/C in cytoplasmic (cyto.) and nuclear (nucl.) fractionated samples to assay the level of contamination...
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Good antibody to detect Notch1
(1)
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Abcam
April 13, 2015
Used this antibody to detect Notch1 protein from whole cell lysates. Antibody recognized a single band at the molecular weight shown from ...
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Good DNA Ladder
(1)
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New England Biolabs
April 13, 2015
This is the go to DNA ladder in our lab when we want to look at sizes between 100 bp and 10,000 bp. It is easy to mix and load onto the gel ...
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Very good p16 antibody for immunoblotting
(1)
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Abcam
April 13, 2015
We are evaluating the basal level of DNA damage markers in wild type and clock deficient mutant mice to understand their role in specific ...
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MUC5AC Immunfluorescence Staining in NHBE
(1)
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Santa Cruz Biotechnology, Inc.
April 13, 2015
This antibody was purchased to use as a marker of goblet cells in airway epithelial cells.
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NR1D1 Antibody used for Western blotting
(1)
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Novus Biologicals, a Bio-Techne Brand
April 10, 2015
We are evaluating changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific pathological ...
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NE-PER Nuclear and cytoplasmic extraction reagent
(1)
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April 10, 2015
This was used to isolate the nuclear extract from the cell lines in order to check the nuclear protein activity in compound treated cells. ...
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Good IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate
(1)
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Thermo Fisher Scientific
April 10, 2015
Primary mouse antibody against acetylated tubulin was detected. It gives a strong signal easily detectable in confocal microscopy and low ...
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E-cadherin / CDH1 Antibody Immunofluorescence
(1)
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Thermo Fisher Scientific
April 10, 2015
Cadherin formation is one of the steps among many in the process of airway epithelial cell differentiation. In our laboratory we use this ...
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Hi Fidelity Polymerase
(1)
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New England Biolabs
April 09, 2015
I used this polymerase while performing PCR to subclone a gene fragment into a viral vector so I could transduce primary epithelial cells. ...
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Very good HA antibody
(1)
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Cell Signaling Technology
April 09, 2015
I used this anti-HA antibody to stain my N-terminal HA tagged NICD3 protein. NICD3 should primarily be in the nucleus and I see nice nuclear...
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Super signal west dura extended duration substrate
(1)
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April 09, 2015
This product was used to check the differential protein expression in proliferation and differentiation of neuronal cells involved in ...
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ZO-1/TJP1 Immunofluorescence
(1)
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Thermo Fisher Scientific
April 09, 2015
In our laboratory we have to track airway epithelial cells throughout differentiation. Tight junction formation is among those steps.
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Secondary Ab donkey anti Rabbit AF555
(1)
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Thermo Fisher Scientific
April 08, 2015
Secondary antibodies from Thermo Fisher Scientific are great for doing immunofluorescence. We work with primary cells (airway epithelial ...
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Ready to use kit for glucose
(1)
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Cayman Chemical
April 08, 2015
I am looking at the effect of eating yogurt before a meal on postprandial blood glucose change. Due to the large quantity of my samples, I ...
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Easy to use for protein quantification
(1)
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April 08, 2015
This product was used in Western blot assays looking at various proteins in the brain homogenate of MPTP-intoxicated animals. After ...
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Rev-erb A alpha/NR1D1 Antibody for Immunohistochemistry
(1)
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Novus Biologicals, a Bio-Techne Brand
April 08, 2015
We are evaluating the changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific ...
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Best HDAC1 antibody
(1)
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April 07, 2015
We are looking at HDACS in macrophages during inflammatory conditions. I chose this product because they have specialized antibodies for ...
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Review for benchtop confocal microscope: EVOS FL Auto system
(1)
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Thermo Fisher Scientific
April 07, 2015
My project involves analysis of the physiological role of the endocytic recycling regulator EHD4 in the kidney. EHD4 is expressed in the ...
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Make sure you get the HS (high sensitive) kit
(1)
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R&D Systems, a Bio-Techne Brand
April 07, 2015
I am working on an intervention study to look at whether yogurt consumption can reduce chronic inflammation, which is linked to the ...
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Galectin-4 Immunohistochemistry Detection
(1)
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R&D Systems, a Bio-Techne Brand
April 07, 2015
Galectin-4 level was shown to be decreased in colon tissues with chronic inflammation and Crohn's disease. Since inflammation is a ...
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Use of EDTA in the passage of BV-2 cells
(1)
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Corning Life Sciences
April 06, 2015
1mM EDTA is used to detach BV-2 cells from a T75 flask.
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Use of Trypan blue stain to count cells
(1)
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April 06, 2015
Trypan Blue is used to stain cells which are then counted on a hemacytometer.
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HER2 Antibody for Immunofluorescence/Western blot
(1)
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Cell Signaling Technology
April 06, 2015
Cell Signaling HER2 antibody #2242 was used for both Western blot and immunofluorescence microscopy to determine the level of HER2 silencing...
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Pay attention to the dilution factor for this kit
(1)
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April 06, 2015
I am interested in the change in EndoCab IgM after 11 weeks of yogurt consumption in humans. My hypothesis is that yogurt consumption can ...
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An easy to use cell counter with accurate results
(1)
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April 03, 2015
We are working with some tough tissue digestion samples, such as skin, and need a way to obtain accurate live cell counts efficiently for ...
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Expression of Phospho-S6 Ribosomal Protein decreased
(1)
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Cell Signaling Technology
April 03, 2015
This antibody was used in a study that investigated the role of Phospho-S6 protein in acute beta-adrenergic receptors antagonist treatment ...
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Quite fast but customer service is lacking
(1)
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Eurofins Medigenomix GmbH
April 03, 2015
We use Eurofins for our DNA sequencing needs. They provide all the necessary supplies (including shipping). We send in the DNA sequence we ...
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Nuclear and cytoplasmic separation of fibroblasts
(1)
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Cell Signaling Technology
April 03, 2015
Nuclear and cytoplasmic proteins from human dermal fibroblasts were separated using salt buffers and centrifugation methods. Separated ...
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Anti-mouse CD40 antibody PercpCy5.5
(1)
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BioLegend
April 02, 2015
The aim of our research is to identify the role of Aryl hydrocarbon receptor activation in dendritic cells and its effects on T cells.
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HILIC for separation of polar metabolites
(1)
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April 02, 2015
The HILIC kinetex column has been great for separating out those residues that just wont separate by RP HPLC. The biggest challenge has been...
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WST-1 reagent for proliferation assays
(1)
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April 02, 2015
This reagent was used to evaluate the proliferation of two sarcoma cell lines, a parental and a clone with acquired resistance, in the ...
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Cell Signaling's VEGFR2 Antibody for Immunofluorescence
(1)
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Cell Signaling Technology
April 02, 2015
This antibody was used in a study that used VEGFR2 as a marker for endothelial cell (EC) differentiation from MSCs. Several antibodies and ...
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Trace Metals for Nematode S-Medium
(1)
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USBiological
April 01, 2015
Our lab uses C. elegans as a model organism to study natural variation in response to stress. To conduct our research, we need many ...
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Quality improved
(1)
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April 01, 2015
I am doing an intervention study regarding intestinal barrier function. One primary outcome is endotoxin level in blood. But as endotoxin (...
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Galectin-4 WB detection
(1)
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R&D Systems, a Bio-Techne Brand
April 01, 2015
Galectin-4 is a tandem galectin that shows high affinity to lipid rafts and has been implicated as a tumor suppressor protein in colorectal ...
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Immunohistochemistry-Paraffin
(1)
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Novus Biologicals, a Bio-Techne Brand
April 01, 2015
Our work focuses on changes in Nr1d1 protein expression in human and mouse lungs. From the available source of antibody we identified this ...
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Micro pH electrode for measuring pH of small samples
(1)
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March 31, 2015
The focus of my project is elucidation of the physiological role of endocytic regulatory protein EHD4 in the kidney. EHD4 is expressed in ...
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Anti-mouse H2kb (MHC I) Pacific Blue antibody
(1)
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BioLegend
March 31, 2015
We used this product to asses effects of Aryl hydrocarbon receptor activation in dendritic cells and T cells. We assessed AhR activation in ...
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BioLegend anti-MHCII FITC antibody
(1)
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BioLegend
March 31, 2015
We used this product to asses effects of Aryl hydrocarbon receptor activation in dendritic cells and T cells. We assessed AhR activation in ...
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Reliable anti-neomycin phosphotransferase type II antibody
(1)
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March 31, 2015
We used the antibody to determine amounts of neomycin phosphotransferase type II (NPTII) from E. coli in samples of transgenic Arabidopsis ...
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Immunoprecipitaiton (IP) Using ITCH Antibody
(1)
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BD Biosciences
March 30, 2015
Immunoprecipitation of ITCH from KB cell extracts using ITCH (BD Biosciences: Cat# 611199) mouse mAb (lane 1) or mouse IgG isotype Control (...
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Mouse anti-V-ATPase antibody
(1)
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Santa Cruz Biotechnology, Inc.
March 30, 2015
My project involves analysis of the physiological role of the endocytic recycling regulator EHD4 in the kidney. EHD4 is expressed in the ...
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Galectin-1 IHC detection
(1)
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R&D Systems, a Bio-Techne Brand
March 30, 2015
Galectin-1 is the prototype of the galectin family and has been implicated in a wide variety of cancers and different tumorigenic processes....
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Reliable, Great Quality, Reproducible Results
(1)
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March 30, 2015
The general aim of our research is to evaluate the role of a metabolic enzyme in generating host-protective Type 2 immunity against ...
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