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Date Published
Dim fluorochrome but highly expressed marker
(1)
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BioLegend
May 04, 2015
We selected CD8a Antibody from Biolegend specifically because CD8a is expressed in T cells and can be found in lymph nodes. Using this ...
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Human Granzyme B ELISA
(1)
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May 01, 2015
My research was to look at Granzyme B production in natural killer cells. Granzymes are serine proteases that are released by cytoplasmic ...
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CellTiter-Blue for use in viability assays
(1)
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Promega
May 01, 2015
CellTiter-Blue was used to evaluate the viability/proliferation of U2OS cells (ATCC) treated with PLK1 or negative control siRNA to ...
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Excellent Human CD45 Antibody
(1)
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BD Biosciences
May 01, 2015
Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. I have used this ...
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Use of MACS separation columns in cell culture
(1)
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Miltenyi Biotec
May 01, 2015
I am conducting research on Parkinson’s disease, looking specifically at how effector T cells are able to modulate and exacerbate the ...
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copGFP Western blot
(1)
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Axxora
April 30, 2015
In our lab we use copGFP as a marker for lentiviral infection, that way we can confirm either by immunofluorescence or Western blot whether ...
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PE/Cy7 anti-mouse CD11c Antibody for Dendritic Cell staining
(1)
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BioLegend
April 30, 2015
Mouse bone marrow was isolated and cultured in vitro with GM-CSF for 5 days, and stained with PE-Cy7 anti-mouse CD11c Antibody.
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Purified anti-mouse CD45
(1)
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April 30, 2015
My research work currently focuses on the relationship between influenza virus infection and type II cells in the lung. Lung type II cell ...
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Just Great CD90.2 (Thy-1.2) Antibody
(1)
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BioLegend
April 30, 2015
The aim of my research is to study lymph node cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion ...
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Very Good Anti-Human HLA-DR antibody
(1)
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BD Biosciences
April 29, 2015
Human PBMC reconstituted NOD-scid IL2r?cnull mice (NSG mice), were sacrificed and single cell suspensions from spleen was used for flow ...
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Good secondary antibody
(1)
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Thermo Fisher Scientific
April 29, 2015
I was using this secondary antibody to detect a primary goat anti-FOXJ1 antibody in primary human bronchial epithelial cells. ...
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Great agarose for DNA gels
(1)
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Lonza
April 29, 2015
We routinely use this agarose to run DNA gels, it melts easily and I have made gels as low as 0.7% as well as very thin gels that hold up to...
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Very Useful CD4 Antibody
(1)
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BioLegend
April 29, 2015
The aim of my research is to study lymph node cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion ...
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Gel Extraction Kit
(1)
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April 28, 2015
Making plasmids is an everyday challenge in research laboratories. A good gel extraction kit is critical for cleaning DNA inserts after ...
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Very Good Anti-Human PD-1 antibody
(1)
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BD Biosciences
April 28, 2015
Human PBMC reconstituted NOD-scid IL2r?cnull mice (NSG mice), were sacrificed and single cell suspensions from spleen was used for flow ...
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Good antibody to detect Actin
(1)
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Abcam
April 28, 2015
Regularly use this antibody to detect actin for loading controls for my western blots. This antibody can be diluted 1:5000 and consistently ...
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Good CD3 Antibody
(1)
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April 28, 2015
The aim of the research is to study bone marrow progenitor cells in cancer. In my experiment, I tested if a cytokine associated with tumor ...
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Immunohistochemical staining of SNpc
(1)
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April 27, 2015
Used for staining of substantia nigra following MPTP intoxication to quantitate surviving dopaminergic neurons. Following incubation of ...
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Use of Life Technologies' DMEM in the culture of BV-2 cells
(1)
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Thermo Fisher Scientific
April 27, 2015
BV-2 cells are cultured and passaged every 3-4 days in fresh complete DMEM.
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Very Good Anti-Human CD8 antibody
(1)
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BD Biosciences
April 27, 2015
NOD-scid IL2rgammac-null mice (NSG mice) reconstitute with human PBMCs were sacrificed and single cell suspensions prepared from spleen was ...
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Good and useful reagent
(1)
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BioLegend
April 27, 2015
The aim of this research is to study bone marrow progenitor cells in cancer. In my experiment, I tested if a cytokine associated with tumor ...
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It extracts DNA but it is not pure
(1)
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QIAGEN - Sample to Insight
April 24, 2015
I used this Gel DNA recovery kit to extract DNA from agarose fragments that were subsequently used for cloning purposes.
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FITC anti-mouse CD11c Antibody for Dendritic Cell Staining
(1)
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BioLegend
April 24, 2015
Mouse bone marrow was isolated and cultured in vitro with GM-CSF for 5 days, and stained with FITC anti-mouse CD11c Antibody.
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Good CD335 (NKp46) Antibody
(1)
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BioLegend
April 24, 2015
We selected NKp46 Antibody from Biolegend specifically because NKp46 is expressed in NK cells and can be found in lymph nodes. Using this ...
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Tryple- Alternative to trypsin
(1)
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Thermo Fisher Scientific
April 24, 2015
This product can be used for very fast dissociation of hard to trypsinize cells without the need to inactivate with FBS or SBTI. The tryple ...
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Good and useful reagent
(1)
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April 23, 2015
The aim of this research is to study bone marrow progenitor cells in cancer. In my experiment, I tested if a cytokine associated with tumor ...
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Very good antibody for detecting human Cd4 expression
(1)
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April 23, 2015
Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. I have used this ...
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Makes marking band sizes easy
(1)
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April 23, 2015
This was used as the ladder when running Western blots to mark molecular weights on the gel. Used with 4-15% gels and transferred to PVDF ...
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Very Good Anti-Human CD45 antibody
(1)
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BD Biosciences
April 23, 2015
Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. I have used this ...
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Good for Duplex PCR
(1)
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Bio-Rad
April 22, 2015
I use this supermix when performing duplex Taqman probe PCR. It amplifies both probes and saves time and sample because I can do duplexing ...
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Very Good Anti-Human CD45 antibody
(1)
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April 22, 2015
Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. This antibody was ...
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Very Good Anti-Human CD3 antibody
(1)
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April 22, 2015
Human PBMC reconstituted NOD-scid IL2r?cnull mice (NSG mice), were sacrificed and single cell suspensions prepared from spleen were used for...
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Good pan-JAK Inhibitor
(1)
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Cayman Chemical
April 22, 2015
I use this compound to inhibit JAK/STAT signaling in my cells. This compound effectively inhibits IL-13 activation of the JAK/STAT pathway, ...
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Purchasing through Quartzy
(1)
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April 21, 2015
Purchasing through Quartzy can potentially save lots of money. It gives you the advantage of potentially finding better prices than the ones...
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Good phospho STAT6 Antibody
(1)
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Cell Signaling Technology
April 21, 2015
I was using the phospho-STAT6 antibody to determine if the IL-13 I purchased was causing activation of the JAK/STAT pathway in primary ...
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Very good antibody for detecting human Cd45 expression
(1)
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April 21, 2015
I have used this antibody to separate human C45+ cells. The staining looks good with two separate populations of CD45 positive and negative ...
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Good for Sybr Green PCR
(1)
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Bio-Rad
April 21, 2015
I was using this to perform quantitative PCR on a cDNA sample or a no template control. The cDNA sample showed amplification but the no ...
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ROR levels in human lung tissue
(1)
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Abcam
April 20, 2015
We are evaluating the changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific ...
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APC anti-mouse CD11c Antibody for Dendritic Cell Staining
(1)
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BioLegend
April 20, 2015
Mouse bone marrow was isolated and cultured in vitro with GM-CSF for 5 days, and stained with APC anti-mouse CD11c Antibody. I used CD11c ...
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Immunofluorescence staining using Life Technologies AF 2nd Ab
(1)
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Thermo Fisher Scientific
April 20, 2015
We do a lot of confocal microscopy in our laboratory. Life technologies is one of our favorite companies to get 2nd antibodies from.
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Ku70 antibody for mouse lung samples
(1)
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Abcam
April 20, 2015
We are evaluating the basal level of DNA damage markers in wild type and clock deficient mutant mice to understand their role in specific ...
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Very Good Anti-Human CD4 antibody
(1)
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April 17, 2015
Human PBMC reconstituted NOD-scid IL2r?cnull mice (NSG mice), were sacrificed and single cell suspensions prepared from the spleens were ...
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Sox11 from US Biological - works well for mouse retina
(1)
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United States Biological
April 17, 2015
This antibody was used in a study investigating the role of transcription factor Sox11 in the adult mouse retina. Several antibodies were ...
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
(1)
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Abcam
April 17, 2015
We are evaluating the changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific ...
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Very good Bmal1 antibody for immunoblotting
(1)
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Abcam
April 16, 2015
We are evaluating the changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific ...
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Easy RNA clean up
(1)
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QIAGEN - Sample to Insight
April 16, 2015
Used this kit to clean up RNA from several sources: tissue, primary neurons, cell lines. The end goal is to run qPCR on these samples and ...
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Good HIF-2alpha antibody
(1)
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April 16, 2015
I was testing to determine if hypoxia increased HIF-2alpha (EPAS1) levels in primary human epithelial cells. I used this antibody to detect ...
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Smoke Robot
(1)
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April 16, 2015
We study the effects of cigarette smoke in airway epithelial cells. This is a great model to expose the cells to whole cigarette smoke (...
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DENV-2 Immunostaining with Anti -dengue Type 2
(1)
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United States Biological
April 15, 2015
Dengue virus (DENV-2) infected HeLa cells were immunostained for P- bodies and DENV-2. Cells grown on cover slips were washed in PBS, fixed ...
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Cotinine ELISA for mouse
(1)
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April 15, 2015
We measure cotinine levels in mouse plasma samples immediatley after exposure to E-cigarettes for 3 days (5Hr/day). Samples between control ...
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