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Date Published
Lympholyte M
(1)
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CEDARLANE
June 12, 2015
We use lympholyte M as centrifugation step in preparation of bone marrow derived dendritic cells.
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Anti-Human IgG1 antibody
(1)
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GeneTex
June 12, 2015
I used this antibody to screen my protein of interest in culture supernatants which had an IgG1 Fc tag. Earlier, with other anti IgG ...
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Isolation of RNA from T cells
(1)
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June 12, 2015
The purpose for using this kit was to isolate RNA from a small number of T cells. I chose it because it seemed easy to use, quick, and it ...
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Transfection quality plasmid purification kit
(1)
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QIAGEN - Sample to Insight
June 12, 2015
We used this kit to purify our plasmid of interest to transfect into mammalian cell lines. This kit provides a high quality plasmid with ...
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Good anti-Mouse CD4 antibody
(1)
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BioLegend
June 11, 2015
The objective of this experiment was to study T cell subsets in mouse spleens. I tested if the anti-CD4 APC/Cy7 antibody from BioLegend can ...
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OCT4 antibody for iPS cells
(1)
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June 11, 2015
I work with human iPS cells and use several antibodies for pluripotency verification. One of them is the OCT4 antibody from Stemgent. I ...
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Vector for TA cloning
(1)
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Promega
June 11, 2015
We use this kit for cloning genes. By using this kit, we can select specific pairs of restriction enzyme sites at both the 5' and 3' ends of...
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Good anti-Mouse CD45 antibody
(1)
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BioLegend
June 11, 2015
We used this antibody to determine CD45 expression in mouse splenocytes. Single cell suspensions isolated from mouse splenocytes were used ...
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CyclinB1 is a very good antibody for Western blot with out any non specificicity
(1)
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June 11, 2015
This product was used in a study to screen anticancer drugs that can inhibit G2/M phase of the cell cycle, in this case CyclinB1 is used as ...
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Co-formulation of a hybrid retroviral gene therapy vector using a fluorescent lipid for applications in microscopy
(1)
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Avanti Polar Lipids Inc.
June 10, 2015
Visualization of gene therapy vectors is critical for quantitative evaluation of their functional performance in various cells. Here, we ...
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Good quality DNA for cloning
(1)
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Illumina
June 10, 2015
We purify DNA from mouse blood for cloning purposes. We tend to get lot of RNA contamination by conventional phenol-chloroform methods. We ...
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If you do a lot of ELISA work, this will be a good companion
(1)
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Agilent Technologies
June 10, 2015
We do a lot of ELISA in our lab. One very tedious part of ELISA is to wash the plate. Not only it takes a lot time, it also wastes a lot of ...
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Menin Antibody for Western Blotting
(1)
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Bethyl Laboratories, Inc.
June 10, 2015
We were interested in looking at the protein levels of menin as well as its interacting partners in prostate cancer cell lines (VCaP and ...
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Recovery of Retroviral Transduction via DOTAP
(1)
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Avanti Polar Lipids Inc.
June 09, 2015
Retroviruses are commonly used gene therapy vectors but can be extremely unstable, inefficient and expensive due to sensitivities in ...
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Accurate quantification of RNA concentration
(1)
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Thermo Fisher Scientific
June 09, 2015
I use this kit for measuring RNA concentration before cDNA synthesis with reverse transcription. Obtaining accurate RNA concentration is ...
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Magnetic Isolation of Regulatory T cells using RosetteSep
(1)
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June 09, 2015
The goal of my research is to determine changes in regulatory T cell populations within the peripheral blood following treatment. I chose to...
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This gel extraction kit has high quality with low price
(1)
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June 09, 2015
This kit contains 250 preparations, the price is low, but the quality is good enough for PCR product recycling and digestion product ...
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Alpha tubulin expression in HELA cells
(1)
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June 09, 2015
This product was used as part of a study to check protein expression changes in cancer. The product worked well as a loading control.
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Plasma Membrane staining to quantify intracellular biochemistry via fluorescence
(1)
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Thermo Fisher Scientific
June 08, 2015
Plasma membrane staining is another way to counterstain for cellular assays apart from nuclear stains. FM 1-43 dye is a highly specific ...
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A must for RNA extraction
(1)
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June 08, 2015
I used this product to extract RNA from peripheral blood mononuclear cells. Since the sample collection took quite a while, I saved my ...
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To measure the HDAC activity/Inhibition
(1)
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EpiGentek
June 08, 2015
This product was used to measure HDAC activity/inhibition in samples treated with HDAC inhibitor.
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DNA isolation using DNeasy kit
(1)
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QIAGEN - Sample to Insight
June 08, 2015
The aim of my research was to understand methylation status on genomic DNA isolated from CD4+ T helper cells. I wanted to use the DNeasy kit...
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Western Blot with phospho-Tuberin S1387 antibody
(1)
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Cell Signaling Technology
June 08, 2015
This antibody was used to detect levels of phospho-tuberin S1387 in cancer cell lines. When loading 50 ug of cell lysate, we were able to ...
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Easy to use for RNA cleanup
(1)
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QIAGEN - Sample to Insight
June 05, 2015
I am interested at the expression level of several inflammatory genes in peripheral blood mononuclear cell. I used this kit for RNA cleanup ...
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Transcriptomics for T cell differentiation
(1)
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June 05, 2015
The aim of my research was to isolate T cells from drug-treated mice and assess genomic changes within the T cell population following ...
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Good anti-Mouse CD45 PE antibody
(1)
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June 05, 2015
The objective of this study was to standardize CD45 staining on mouse splenocytes. For this purpose single cell suspensions were isolated ...
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Efficient Nuclear Labeling of Live Cells
(1)
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Thermo Fisher Scientific
June 05, 2015
Primary tissues and cells are routinely analyzed for various biochemical and pharmacological behavior. One of the primary methods to ...
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HDAC2 expression in N2A cells
(1)
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Abcam
June 05, 2015
We do work on gene and protein expression changes (epigenetic modulators) in neuroglia differentiation and behaviour. This product was used ...
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Tonbo CD11c Red Fluor 710 antibody
(1)
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June 04, 2015
I used Tonbo CD11c for assessment of bone marrow-derived dendritic cells via fluorescence cytometry.
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HDAC1 expression in N2A cells
(1)
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Abcam
June 04, 2015
We do work on gene and protein expression changes (epigenetic modulators) in neuroglia differentiation and behaviour. We used this product ...
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Great F4/80 Antibody
(1)
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BioLegend
June 04, 2015
I use this antibody for labeling Kupffer cells from murine livers. On a BD LSRII, BD Aria IIu, or a BD Canto II, I see my cell population ...
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Permeabilization kit for intracellular staining of FoxP3
(1)
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June 04, 2015
The specific aim for using this product was to permeabilize T cell membranes to allow intracellular detection of FoxP3 using fluorescent ...
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Human sCD14 ELISA
(1)
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R&D Systems, a Bio-Techne Brand
June 04, 2015
I am looking at whether yogurt consumption could improve intestinal barrier function in an obese population. Compromised barrier function ...
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Streptavidin Cy5 for biotinylated aptamers
(1)
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June 03, 2015
Our laboratory selects aptamers for various cell receptors. To test the specificity of aptamer binding to white blood cells or transfected ...
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H3 pan as loading control for checking histone modifications
(1)
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MilliporeSigma
June 03, 2015
We used this product to check the histone acetylation in glioblastoma cells treated with anticancer drugs.
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PD98059 to check MAPK/ERK signaling
(1)
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MilliporeSigma
June 03, 2015
This product was used to check the molecular signaling of neurotrophic compounds. The product inhibits MAK1/MEK2 and is useful for studying ...
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Affordable kit for plasma TG
(1)
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June 03, 2015
I am interested at postprandial change in TG level after a co-ingestion of yogurt and high calorie meal. Hourly blood samples were collected...
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FZD7 Abs to verify the transfection of HEK 293 cells
(1)
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Novus Biologicals, a Bio-Techne Brand
June 03, 2015
HEK 293 cells were transfected with FZD7 and later used for aptamer selection. Abs were used to verify the transfection of HEK 293 cells.
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Streptavidin APC for biotinylated aptamers
(1)
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June 02, 2015
Anti-S1PR1 biotinylated aptamers were used to stain white blood cells. Aptamers were first incubated with Streptavidin APC and then added to...
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PD-L1 expression in bone marrow derived DCs
(1)
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BioLegend
June 02, 2015
We are assessing the expression of PD-L1 (CD274) on bone marrow derived dendritic cells.
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Reliable Human TNF-RII ELISA kit
(1)
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R&D Systems, a Bio-Techne Brand
June 02, 2015
I am interested in whether yogurt consumption could decrease chronic inflammation in obese populations. Previous reports have shown a ...
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cDNA conversion for RT-PCR application
(1)
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Thermo Fisher Scientific
June 02, 2015
The aim of my study was to determine gene changes associated with T cell profiles using RT-PCR. Specifically, I wanted to use this product ...
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Acetyl H3 expression in GL-261
(1)
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MilliporeSigma
June 02, 2015
We used this product to check the expression of Acetyl H3 in Gl-261 treated with anticancer compounds. This product works relatively well.
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Good IgM Antibody
(1)
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BioLegend
June 01, 2015
We selected IgM Antibody from Biolegend specifically because IgM is expressed in B cells and can be found in lymph nodes. Using this product...
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Better options out there for this dim fluorochrome
(1)
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June 01, 2015
I needed to design a 10-color staining panel on T cell markers for the LSRII and was looking for an antibody conjugated to the Alexa700.
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Acetyl histone H4 expression in HDACI treated GL-261 cells
(1)
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MilliporeSigma
June 01, 2015
This product was used to check the differential acetyl H4 expression in hdaci treated Mouse glioma (GL261) cells. This product works well to...
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Detecting transcription factor Foxp3 (mouse) easily
(1)
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June 01, 2015
Regulatory T cells that express Foxp3 and CD25 (Tregs) are crucial during immune homeostasis, and are involved in many diseases such as ...
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Good and Useful CD4 Antibody
(1)
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BioLegend
June 01, 2015
We selected CD4 Antibody from Biolegend specifically because CD4 is expressed in T cells and can be found in lymph nodes. Using this product...
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BDNF expression in neuronal differentiation and neurotrophicity
(1)
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Abcam
May 29, 2015
This product was used to find BDNF expression and monitor changes in neural differentiation and neurotrophicity. The product is effective in...
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IDT has the best DNA/RNA oligos
(1)
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Integrated DNA Technologies
May 29, 2015
We have been ordering oligos from IDT for a long time now. 99% of the oligos work very well.
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