Enzo Life Sciences
Total ROS detection kit for microscopy and flow cytometry
ENZ-51011
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I work on trying to determine the source of ROS production in eosinophils following the engagement of a eosinophil specific surface receptor. I used this kit to understand the kinetics of ROS following receptor engagements. When looking at potential kits, I decided on this one because it had all the reagents necessary and the protocol was simple.
Flow Cytometry
Human peripheral blood eosinophils
I followed the exact protocol provided by the kit. Cell stimulus was added in normal media, which I washed off with buffer provided by the kit.
If you are using a cell type that can easily degranulate/activate in tissue culture coated plates, please coat your plates with something like fibronectin. This will save you a lot of time
I used flow cytometry and analyzed the data using geometric MFI. Data can also be analyzed using % ROS production, which can be tricky because the gating can be difficult.
None
Easy to use and quick
Nothing really.
This is a great kit, easy to use, reliable, and fast.
Product Name: Total ROS Detection KitFor Microscopy and Flow CytometrySupplier Page