Fisher Scientific
Harris modified Hematoxylin and Eosin solution
SH26-500D & CAT#314-630
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This is a very good kit for H&E staining, the nuclear staining is strong enough to separated and the color is very nice, especially for the Eosin staining. The bottle can be used for a long time and can be used for staining of several thousands of slides.
Pathology
Various
H&E Stain ---new Kit Components: Mayer’s Hematoxylin Solution 250 ml FROM FISHER Eosin Solution 250 ml 1% Acid Alcohol 250ml (2.5ml HCl + 250ml 70% EtOH) Acetic Acid Glacial 1ml Eosin Solution + 2ul Acetic AcidProcedure: For Paraffin Sections: 1. Deparaffinize sections with xylene, 5 min ×2 2. Re-hydrate with 100% alcohol, 2 min ×2 3. Re-hydrate with 95% alcohol, 2 min ×2 4. Rinse in running tap water for 2 min 5. Stain in Mayer’s Hematoxylin Solution for 30 seconds 6. Rinse in running tap water for 2 min 7. Dip in 1% Acid Alcohol ×2 8. Wash with running tap water for 2 min 9. Counterstain in Eosin Solution (1ml Eosin Solution + 2ul Acetic Acid Glacial) for 2 -3 min 10. Rinse with 3—4 washes of tap water 11. Rinse quickly in 95% alcohol (20 dips ×2). 12. Dehydrate through 100% alcohol (10 dips ×2). 13. Clear in xylene, 2 min ×2 14. Add 2-3 small drops of Cytoseal, Slowly apply coverslip, avoiding air bubbles.
Just increase staining time if you want to use slides for a long time.
Very good staining kit
None
Works well
Eosin staining time is 10-30 seconds