Affymetrix eBioscience
Mouse IgG2a K Isotype Control eFluor® 450
48-4724-80
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Peritoneal macrophages can be easily sorted and analyzed via flow cytometry using the pan-macrophage marker - F4/80. However, to confirm and tightly control the gating, isotype controls are important. This product provides a very good negative control to analyze the F4/80 population accurately.
Flow cytometry
Peritoneal lavage from mice
Standard flow cytometry protocols were used. Cells were incubated with the antibody at 1 ug/100,000 cells at 1:10 volumetric ratio for 30 mins in the dark at 4 deg C. Cells were centrifuged and resuspended in a 300-500 ul of PBS + 2% serum.
Use clean flow cytometry tubes. Accurate pipetting is important.
As seen in the image below, the isotype controls were slightly positive as compared to unstained cells while still low enough to mark out the F4/80+ sample. This clearly proves that isotype controls are important to setup the gates for the F4/80 analysis and sorting.
None
Very easy to use and highly accurate
Isotype controls such as this are vital to obtain accurate cell sorting and analysis
Product Name: Mouse IgG2a K Isotype Control eFluor® 450 25 ugSupplier Page