BACH2 polyclonal antibody for IHC

Academic Haematology
Newcastle University
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Atlas Antibodies

Product Name:

BACH2 antibody

Catalog Number:

HPA051384

We are interested in BACH2 for its role in B cell maturation. I have used the BACH2 antibody to assess BACH2 protein expression in high grade B-cell lymphoma samples. Specifically, this was performed by immunohistochemistry on formalin fixed paraffin embedded tissue sections.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

FFPE tumour biopsies

Primary Incubation

BACH2 antibody was applied for 30 minutes at a 1:100 dilution

Blocking Agent

The antibody was run in conjunction with the Ventana UltraView Universal DAB Detection Kit

Secondary Incubation

The antibody was run in conjunction with the Ventana UltraView Universal DAB Detection Kit which incorporates a cocktail of enzyme labelled secondary antibodies

Tertiary Incubation

The antibody was run in conjunction with the Ventana UltraView Universal DAB Detection Kit.

Detection

Hydrogen peroxide substrate and 3, 3’-diaminobenzidine tetrahydrochloride (DAB) chromogen

Results Summary

Results show clear staining of BACH2 positive tumour cells. Both cytoplasmic and nuclear staining can be detected.

Additional Notes

None

Related Categories

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Summary

The Good

Clear positive staining for BACH2

The Bad

Requires some optimisation

The Bottom Line

Reliable antibody for clear staining of BACH2 protein

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