Takara Clontech
Retrovirus Titration by qRT-PCR
631453
Retroviral quantification is critical especially when combined with other biomolecular assays for precise control of signaling mechanisms. This kit provides a quick and easy way to quantify viral titer to remove bottle-necks in terms of troubleshooting and confirm viral producer cell performance.
Retroviral Transduction
Viral RNA from producer cell line. See http://www.clontech.com/US/Products/Viral_Transduction/Retroviral_Packaging/High-Titer_Retrovirus & https://www.qiagen.com/us/products/catalog/sample-technologies/rna-sample-technologies/viral-rna/qiaamp-viral-rna-mini-kit/
Exact protocol from the manufacturer was used. No other optimization is needed as the primers and RNA standards are available in the kit.
Extremely critical to have lab benches clean from genetic contamination
Close to 100% PCR amplification is obtained as confirmed via the image. No dual sequence amplification as confirmed via the dissociation curves.
None
Very quick assay for confirming retroviral titer
Quick (within 4 hours) titer of Retroviral titer is possible via the use of this kit. Multiple steps are combined into 1 step due to the standards being mRNA themselves.