Qiagen
QIAamp Viral RNA Mini Kit
52904
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Producer cell lines to obtain retroviruses and adenoviruses are commonly used in most viral therapeutics and transduction labs. In order to precisely quantify the number of viral particles, efficient viral RNA isolation is required which was easily done via this kit.
Quantification of viral particles
Cell supernatant from producer cell lines - See http://www.clontech.com/US/Products/Viral_Transduction/Retroviral_Packaging/High-Titer_Retrovirus
Protocol suggested by the manufacturer was used. No other optimization was done.
Make sure lab bench is extremely clean to avoid other cellular contamination
Isolation was a breeze with this kit. The attached image shows the viral rna isolated following reverse transcription, traditional pcr, and gel electrophoresis. Lane 1 shows the ladder and Lanes 2 and 3 show our cDNA for the RNA which we were interested in. For reverse transcription, we used the following kit - http://www.lifetechnologies.com/order/catalog/product/N8080234
None
Quick and easy
Highly repeatable and precise RNA isolation of viral particles
Product Name: QIAamp Viral RNA Mini Kit from QIAGENSupplier Page