Easy kit for plasmid midipreps

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Overall

Quality of Results

Ease-of-Optimization

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Company:

Omega Bio-Tek

Product Name:

E.Z.N.A FastFIlter Plasmid DNA Midi Kit

Catalog Number:

D6905-03

I perform a lot of cloning in the lab and use this kit to isolate plasmid DNA from bacteria. I like using this kit because it is pretty easy to use and quick. The DNA yield is good and successfully used with downstream applications such as cloning. It is also a little cheaper than other kits to my knowledge.

Experimental Design and Results Summary

Application

Plasmid Isolation

Starting Material

None

Protocol Overview

Grow 50 ml LB + bacteria overnight. Spin down bacteria to a pellet. Follow midiprep protocol and isolate DNA

Tips

Follow protocol

Results Summary

The kit yields hundreds of nanograms per microliter concentration of plasmid DNA with good spec. readings

Additional Notes

None

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Summary

The Good

Good yield and easy kit

The Bad

None

The Bottom Line

This is good kit for plasmid isolation.

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