Great OSMR Primer/Probe Set

Northwestern University
Medecine
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher (Applied Biosystems)

Product Name:

Oncostatin M Receptor primer/probe set

Catalog Number:

Hs00384276.m1

We research chronic sinus disease and thus use human sinus tissue. This primer/probe set for OSMR is great.

Experimental Design and Results Summary

Application

RT-PCR

Starting Material

Human sinus tissue lysates

Protocol Overview

This is a 20x solution, so if your reaction is 20uL, then you only need 1uL per reaction

Tips

Applied Biosystems offers an xs size so you can try it for under $100

Results Summary

Results were always reliable using this primer/probe set.

Additional Notes

Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease. Pothoven KL, Norton JE, Hulse KE, Suh LA, Carter RG, Rocci E, Harris KE, Shintani-Smith S, Conley DB, Chandra RK, Liu MC, Kato A, Gonsalves N, Grammer LC 3rd, Peters AT, Kern RC, Bryce PJ, Tan BK, Schleimer RP. J Allergy Clin Immunol. 2015 Sep;136(3):737-746.e4. doi: 10.1016/j.jaci.2015.01.043.

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Summary

The Good

Great primer/probe set, super easy, highly recommended

The Bad

More expensive than sequencing primers and probes

The Bottom Line

You should use this primer probe set.

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