Good Staining for Gamma Delta T Cells

Pulmonary
Harvard Medical School
Post Doc

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Company:

BD Biosciences

Product Name:

Anti-TCR-gamma delta; PE Clone11F2

Catalog Number:

333141

Browse Similar Products: TCR Flow Cytometry Antibodies

The aim of this research project was a phenotypic analysis of human peripheral blood using minimal amounts of samples. Conventional as well as gamma delta T cells were identified using BD antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human peripheral blood

Primary Incubation

Peripheral blood was incubated for 30 minutes with the antibody in the dark at room temperature. The blood was lysed using BD lysing buffer as indicated in the instructions. Then sample was washed 3 times with PBS and resuspended in 300ul of FACS buffer for direct flow cytometry

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

This antibody provides a nice staining of TCR gamma delta positive cells. Cells can be separated form conventional CD4+ and CD8+ T cells in a very good way.

Additional Notes

In the image you see lymphocytes stained with a CD4 and the TCR gamma delta antibody.

Related Categories

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Summary

The Good

Very good separation of TCR gamma delta T cells

The Bad

None

The Bottom Line

This antibody provides a very good separation of TCR gamma delta positive and negative cells

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