Life Technologies (Thermo Fisher Scientific)
N-2 Supplement (100X)
17502-048
This product was used to culture glioma stem cells from glioblastoma cells (U87). The product supports glioma cell growth in serum-free neural stem media.
Inducing the Glioma cancer stem cells from glioblastoma cells (U87 cells)
U87 cells
For 10mL - DMEM/F12-9mL,540uL, N2 supplement (100x)-0.1mL, B27 supplement (50x)- 0.2mL, bFGF (10ug/mL)-20uL, EGF (10ug/mL)-20uL, Penstrep-0.1ml, BSA (250MG/mL)-20uL. Mix it and filter it with syringe filter (0.22micron).
The growth factors are unstable, make required amount of media every time.
The glioma cancer stem cells were induced from glioblastoma cells, confirmed with stem cell marker expression in immunoblot.
None
Supports the growth of glioma stem cells in serum free media.
Nothing bad about the product.
Recommended to use for culturing glioma stem cells in serum-free neural stem cell media.
Product Name: N-2 Supplement (100X)Supplier Page