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Alexa Fluor® 594 anti-rat IgG
405422
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To detect mouse intestinal cells by IFA, intestinal tissue was collected from mice, then snap frozen and cryo sections were used for staining the intestinal epithelial cells. Sections were fixed and then blocked. Then, sections were stained with Anti-EpCAM antibody followed by Alexa Fluor® 594 anti-rat IgG staining. Slides were coverslipped with DAPI containing mounting medium and analyzed under fluorescence microscopy. Strong specific signals (positive cells) could be found under fluorescence microscopy.
Immunofluorescence
Mouse intestinal tissue
Anti-EpCAM antibody
5% FBS in PBS
NA
Fluorescence microscopy
Intestinal epithelial cells were stained with very strong and specific signal. The secondary antibody did not cross-react with any other protein of the tissue sample. Thus, we were able to clearly visualize the intestinal epthelia layer.
Can be used at high dilution rate. Its cost efficient.
Sensitive and can generate strong fluorescent signal
None
This antibody generated very strong and specific signals and was very good for IFA experiment.