Detection of CD4 Positive T Cells in Mouse Breast Cancer Tumors

Pathology
The Ohio State university
Post-docotoral researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 488 anti-mouse CD4 Antibody

Catalog Number:

100425

The orthotopic syngeneic mouse tumors established using MVT1 cell line were analyzed for the presence of CD4+/CD3+ T cells using Alexa Fluor® 488 anti-mouse CD4 Antibody. The tumors were pulverized and single cells were prepared. The cells were stained with Alexa Fluor® 488 anti-mouse CD4 Antibody and analyzed using flow cytometry. The Alexa Fluor® 488 anti-mouse CD4 Antibody successfully detected the T cell population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse mammary gland tumors

Primary Incubation

30 minutes on ice

Blocking Agent

5% FBS in TBS buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

The BioLegend CD4 antibody (Cat# 100425) successfully detects the CD3+/CD4+ T cells.

Additional Notes

None

Related Categories

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Summary

The Good

Quick and easy to use

The Bad

None

The Bottom Line

Very good antibody to analyze T cells using flow cytometry

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