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PE Cy7 anti-mouse I-A/I-E Antibody
107629
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Class-II MHC is expressed on all antigen presenting cells including DCs, macrophages and B cells. The expression increases during an immune response. I used this antibody to test MHC-II expression on macrophages. While the expression was positive, it was smeared and messy. On B cells, the expression was very high.
Flow Cytometry
Lymphocytes
1:400 in PBS for 30 minutes
FcR block
NA
Flow Cytometry - PECy7 channel
The expression is positive but non-uniform and smeared. It may be better to titrate or use a different fluorophore.
None
Very bright staining
Smeared and messy
Good for staining, but prefer a different fluorophore