Good Staining to Identify CD45+ Cells in Human Peripheral Blood

Primary Incubation

Staining was performed directly on 50ul of peripheral blood. 1ul of antibody was used and incubated for 30 minutes at room temperature. Cells were then lysed with 1ml of BD lysing solution. After that cells were washed three times with PBS and then resuspended in 300ul of FACS buffer. Cells were directly taken to measure using flow cytometry