FITC Anti-Mouse B220 Ab Used for Labeling Mouse B cells

November 19, 2015

Internal Medicine.
Rush University Medical Center
Research Assistant

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

FITC anti-mouse/human CD45R/B220

Catalog Number:

103206

Our aim is to gate mouse B cell populations for downstream research. B220 is a common cell marker for B cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Splenocytes

Primary Incubation

1X10^6 suspension cells were incubated with 0.5ul FITC anti-mouse CD45R/B220 in 50ul FACS buffer for 30 min at 4oC.

Blocking Agent

0.5ul CD16/CD32

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

Good antibody

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

It is easy to use.

The Bad

None

The Bottom Line

It is a good antibody for mouse B cells.

Share your experience with other scientists. Write a Review! »

Join the discussion