Zeiss Axio Imager 2 Fluorescence Microscope with Apotome

Vanderbilt University Medical Center
Molecular Physiology and Biophysics
Postdoctoral Research Fellow

Overall

Performance

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Zeiss

Product Name:

Axio Imager 2 fluorescence microcope with Apotome

Catalog Number:

Axio Imager M.2

Zeiss AxioImager M2 with the apotome system is utilized for fluorescence and bright field Imaging. The use of the Apotome creates optical sections of your fluorescent samples resulting in near-confocal resolution and crisp images. I have used this scope for three years now.

Experimental Design and Results Summary

Application

Fluorescence microscopy

Starting Material

None

Protocol Overview

The Zeiss Axio Imager M.2 microscope is utilized to capture fluorescent images from immunostained tissue samples.

Tips

Receive training from a Zeiss expert before using the microscope. There are unique features about the Apotome that need to be learned

Results Summary

Overall, this Zeiss fluorescent scope is an outstanding microscope. The use of the Apotome creates very clear and crisp images as long as all the parameters are set properly.

Features Summary

Apotome creates near-confocal resolution images

Additional Notes

Image shows immunofluorescence staining of pancreatic mouse tissue embedded in OCT.  Pdx1 (Red), Insulin (Green), Glucagon (White), DAPI (Blue).

Image Gallery

Summary

The Good

Ease of use. Clear, crisp images

The Bad

None

The Bottom Line

This is an excellent microscope to perform immunofluorescence imaging on stained tissue sections.

Join the discussion