R&D Systems
Mouse/Rat IL-33 Quantikine ELISA Kit
M3300
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I study release of various cytokines by immune cells in response to antigens in vitro as well as in vivo.
Cytokine ELISA
Cell culture supernatants
Culture supernatants were incubated overnight at 4 degrees, followed by washings with wash buffer and HRP conjugated antibody. ELISA was revealed by color solution A and B.
Standardization is necessary to work out the right dilution for supernatants
Cytokine release was detected in response to various antigens
None
Gives very clean results
Expensive
mIL33 release was detected in response to various antigens.
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