Good SPARC Flow Antibody

Neurology
University of Michigan
Research Lab Specialist

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

SPARC, PE conj

Catalog Number:

IC942P

Antibody worked fantastically with isolated murine central nervous system cells, identifying glial cells expressing SPARC with a high enough MFI to compare an increase or decrease with differing disease conditions or treatments. There weren't many choices of conjugated flow SPARC antibodies, and we know R&D antibodies generally work well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse CNS

Primary Incubation

30min, RT, 1:50

Blocking Agent

FC block, 50ug/mL

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

PE

Results Summary

Decent separation, decent MFI

Additional Notes

None

Related Categories

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Summary

The Good

Pre-conjugated

The Bad

High concentration necessary

The Bottom Line

Works

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