Excellent Caspase 3 Antibody

November 20, 2015

Department of Microbiology and Immunology
University of Illinois, Chicago
PostDoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Cleaved Caspase 3 (Asp175)

Catalog Number:

9661

Info: View Product View Product Specs

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I check apoptosis induction in 293T cells by western blot. This antibody works perfectly with no background, it is highly specific and reproducible.

Experimental Design and Results Summary

Applications

Western Blot

Sample

293T whole cell lysate

Primary Incubation

Cleaved caspase 3 antibody diluted in 5% non fat milk in TBST, incubated O/N at 4 degrees

Blocking Agent

5% non fat dry milk

Secondary Incubation

Anti-rabbit secondary antibody

Tertiary Incubation

NA

Detection

ECL

Results Summary

Great antibody to work with. It gives a single band with no background.

Additional Notes

None

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Summary

The Good

Specificity

The Bad

None

The Bottom Line

It works every time for all my westerns. It's reliable and a great antibody.

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