Excellent Antibody for DAP12 Staining

November 16, 2015

BlueskyReddit
Internal Medicine
University of California
Assistant Project Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Beckman Coulter, Inc.

Product Name:

Anti DAP12 PE

Catalog Number:

PN A78886

Browse Similar Products: DAP12 Flow Cytometry Antibodies

This product was used to identify intracellular DAP-12 expressing cells in various myeloid subsets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

20ul per million cells for 2 hrs in the dark

Blocking Agent

Human AB serum

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

PE conjugated on the antibody on FACS ARIA

Results Summary

Very sensitive and good signal

Additional Notes

None

Image Gallery

Summary

The Good

Signal to noise ratio is good

The Bad

Expensive

The Bottom Line

Overall good antibody at expensive price though

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