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Date Published
ELISA Works Great and is Very Specific
(1)
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IBL- America
January 25, 2023
I was surprised at how well this ELISA worked. The method was as simple as most ELISAs are. What was striking is how specific it is. In ...
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Ideal Kit for Extracting Nuclear and Cytoplasmic Protein
(1)
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Thermo Fisher Scientific
January 25, 2023
The kit was used to extract nuclear and cytoplasmic protein in cultured HEK293 cells. The protocol is very easy to follow up, and the ...
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Not My First Choice for coIP
(1)
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January 25, 2023
I used these dynabeads to attempt coIP. However, I ran into several troubleshooting problems. The main issue I had was that the antibody I ...
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"Super Sensitive" is in the Substrate's Name
(1)
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Thermo Fisher Scientific
January 25, 2023
I use this for western blot detection. It is more expensive than other ECL reagents, but the price is worth it. My peers expose their blots ...
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S6 Ribosomal Protein Antibody Works Well for Western Blot
(1)
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Cell Signaling Technology
January 24, 2023
The cells (macrophage) were seeded for 24 hours in 10% FBS RPMI. The following day were stimulated with tgf-b (5 ng/ml) for 24 hours in ...
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Great T Cell Antibody for Flow Cytometry
(1)
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BioLegend
January 24, 2023
T cell infiltration into the brain during respiratory infection in mice was measured by staining for CD3 and analyzed by flow cytometry.
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First Recommendation for coIP
(1)
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January 24, 2023
I used these beads to perform coIP in cell lysates. I like these magnetic beads because they do not use an antibody to capture your protein ...
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Good Anti-CTLA-4 Antibody in Humans
(1)
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BioLegend
January 24, 2023
Our research aims to understand how some CD4 T cells become autoreactive in melanoma patients treated with check point inhibitors. In this ...
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Great Mouse Anti-Human IgA1-PE from SouthernBiotech
(1)
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SouthernBiotech
January 24, 2023
We are an immunotherapy focused group, with interest in CD8 T-cells and their TCRs. For our experimental purposes, we were interested in ...
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HA Tag Antibody from ProteinTech Never Fails
(1)
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Proteintech Group Inc
January 24, 2023
At this rate, ProteinTech is going to get a line in the acknowledgments of my dissertation. I have never had a bad antibody from them. This ...
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Excellent Antibody to Detect Collagen I
(1)
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Abcam
January 23, 2023
We used this antibody to detect Collagen I levels in mammalian cell lysates. The antibody worked really well. The antibody is very specific....
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Amazing Technique to Show Protein Interactions
(1)
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MilliporeSigma
January 23, 2023
I love this kit and technique to show protein-protein interactions. It formed the basis of my most recent publication. The kit is very easy ...
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Reliable and Strong CD56 for Human NK Cells
(1)
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BioLegend
January 23, 2023
We work on NK subpopulations of the human immunosystem. We use this antibody in coupled with different fluorophores to identify the whole ...
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Good X-treme Gene 9 DNA Transfection Reagent from Roche
(1)
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Roche
January 23, 2023
We are an immunotherapy focused group, with interest in CD8 T-cells and their TCRs. For our experimental purposes, we routinely transduce ...
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Great Hoechst 33342 Solution for Live Cell Staining
(1)
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Thermo Fisher Scientific
January 23, 2023
Used this product at a final concentration of 1mg/ml, to stain the nuclei of living cells during imaging.
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Anti-TDP43 Antibody for Western Blot
(1)
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Abcam
January 23, 2023
We have used this antibody to detect TDP43 levels in mammalian cell lysates. The antibody is very specific and works well at the suggested ...
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Great Phospho-p44/42 MAPK Antibody for Western Blotting
(1)
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Cell Signaling Technology
January 20, 2023
Used this antibody as a MAPK signalling downstream of the EGF receptor in MCF10a cells. The antibody has very high signal so use quite ...
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Great Caveolin-1 Antibody for Flow Cytometry
(1)
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Cell Signaling Technology
January 20, 2023
Changes in Caveolin-1 expression brain endothelial cells in response to respiratory infection was measured by flow cytometry.
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Recommended RAS Antibody from CST for Western Blot
(1)
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Cell Signaling Technology
January 20, 2023
Epithelial cells were seeded in 10 % FBS F-12 k. The day after the medium was replaced with 0.5% FBS and stimulated with PDGF (10 ng/ml) for...
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Easy Cloning with SOC Outgrowth Medium
(1)
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New England Biolabs
January 20, 2023
I use SOC Outgrowth Medium to allow my bacteria to recover and give them time to express the antibiotic gene resistance after transformation...
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Great RNase Away from Thermo Scientific
(1)
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Fisher Scientific
January 20, 2023
Works great to wipe surfaces to eliminate RNase when working with RNase sensitive samples. We use this when working with RNA extractions and...
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Great Kit for Detecting LDH Activity in Samples
(1)
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January 20, 2023
We used this kit to measure LDH activity in the mammalian samples to quantitate cell death after specific drug treatment.
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Decent lgBiT-Antibody for Western Blot
(1)
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Promega
January 19, 2023
This monoclonal mouse antibody against the large subunit of the Promega-developed split-luciferase NanoBiT works well for detection via ...
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Reliable and Easy Option to Stain for TNF-a in Human
(1)
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BioLegend
January 19, 2023
Our research aims to understand how some CD4 T cells become autoreactive in melanoma patients treated with check point inhibitors. In this ...
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Easy Plasmid Purification
(1)
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January 19, 2023
I use this kit to perform plasmid DNA maxiprep. I obtain high-quality DNA that can be used for transfection.
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Good Gel Filtration Markers Kit
(1)
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January 19, 2023
We used the protein standards from this kit for our gel filtration experiment to calculate the protein molecular weight. In the column, ...
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Cleaved PARP Antibody for Western Blot
(1)
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Cell Signaling Technology
January 19, 2023
I used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 ...
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Reasonably Good Antibody to Detect GFP-Tag
(1)
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MilliporeSigma
January 19, 2023
We used this antibody to detect GFP tags in western blot applications. The antibody is not highly specific and works well at the suggested ...
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Good TGF-b1 Protein (240-B)
(1)
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R&D Systems, a Bio-Techne Brand
January 18, 2023
To induce EMT for BT20 cells, 10 ng/ml TGF-b1 was administered for incubation of 72 h. The cells were collected for fractionation using NE-...
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Great CD3epsilon Antibody for Flow
(1)
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BioLegend
January 18, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Fast and Easy Miniprep Kit
(1)
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New England Biolabs
January 18, 2023
This kit allows quick and easy purification of high quality plasmid DNA, that can be used to transfect cells.
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Good Phospho-S6 Ribosomal Protein mAb for Western Blot
(1)
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Cell Signaling Technology
January 18, 2023
I used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 ...
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Good Anti-Mouse CD23 BV711
(1)
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BD Biosciences
January 18, 2023
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against ...
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HLA-A2 Staining
(1)
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BioLegend
January 18, 2023
I use this anti-HLA-A2 antibody to determine the HLA phenotype of human T cells. Fluorescent signal is good, allowing an easy distinction ...
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Fixable Viability Dye for Dead Cell Staining
(1)
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January 17, 2023
This viability dye irreversibly labels dead cells. I use it to exclude dead cells from FACS analysis.
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Reliable and Bright Option to Stain HLA-DR
(1)
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BD Biosciences
January 17, 2023
Our research aims to understand how some CD4 and CD8 T cells become autoreactive in melanoma patients treated with check point inhibitors. ...
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Perfect MAPK Antibody
(1)
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Cell Signaling Technology
January 17, 2023
Band was detected at 40kDA after incubating the membrane overnight at 4 °C. Cells were induced with TGF-b for 48 hours in starvation medium ...
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Super Bright and Economical CD62L Antibody
(1)
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BioLegend
January 17, 2023
I used this antibody to characterize T cell activation and differentiation in C57BL/6 mice. I used this antibody on the murine model of ...
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Versatile PD-1 Antibody Detectable on Most Flow Cytometers
(1)
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BioLegend
January 17, 2023
I wanted to study T cell exhaustion/ regulation in mice during the infection with Plasmodium berghei ANKA, a murine model for malaria. I ...
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CD3 Antibody for T Cell Panels
(1)
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BD Biosciences
January 17, 2023
I used this antibody to identify T cells in C57BL/6 mice in several organs, to further study Tr1 cells and other subsets.
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CD44 Antibody with Little Spillover
(1)
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BD Biosciences
January 17, 2023
I used this antibody to analyze T cell differentiation in a panel also B cells, monocytes, DCs and NK cells in C57BL/6 mice infected with ...
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DNA Gel Extraction with Monarch Buffer
(1)
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New England Biolabs
January 13, 2023
I use this buffer to extract DNA fragment from agarose gel. This buffer allows the dissolution of the agarose slice containing the DNA ...
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Great Ly-6G/Ly-6C Antibody for Flow
(1)
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BioLegend
January 13, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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EGF (236-EG) Protein Works Well
(1)
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R&D Systems, a Bio-Techne Brand
January 13, 2023
To induce EMT for BT20 cells, 20 ng/ml EGF was administered for incubation of 72 h. The cells were collected for fractionation using NE-PER ...
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Anti-RUNX1 Antibody
(1)
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R&D Systems, a Bio-Techne Brand
January 13, 2023
To examine the expression of different RUNX1 fusion proteins caused by intragenic rearrangements, antibodies produced from different ...
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Great CD11b Antibody for Flow
(1)
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BioLegend
January 13, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Generating Chemically Competent E.coli
(1)
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January 13, 2023
This kit is designed for the generation of chemically competent E.coli without heat shocking, but I find it is still necessary to perform a ...
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PD-L1 Antibody is Detectable on Multiple Cell Types
(1)
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Thermo Fisher Scientific
January 12, 2023
I tested this antibody to identify co-inhibitory molecules and their ligands on T cells in a large panel which also analyses B cells, ...
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Cryopreservation with Synth-A-Freeze Medium
(1)
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January 12, 2023
Really great medium to cryopreserve primary human cells lines, such as lymphocytes. Cell viability and recovery after thawing is truly ...
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Great LAG-3 Antibody for the Detection of Tr1 Cells
(1)
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BioLegend
January 12, 2023
I used this antibody to characterize and sort Tr1 cells (identified as CD49b+ LAG-3+ CD4 T cells) in mice infected with Plasmodium berghei ...
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ELISA Kits
Enzymes
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Incubator
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Kits
Labels / Dyes / Stains
Ladders and Markers
Light Sources
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Western Blot
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ELISA
Immunofluorescence
Immunoprecipitation
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Block/Neutralize/Inhibit
Cell Isolation
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Staining
Cell Culture
DNA Purification
PCR
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RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
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Cell Counting
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Library Construction
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Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
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Horse
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C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
Squirrel
Fish
Virus
Other Invertebrate
Xenopus/Amphibian
Drosophila
Goat
Rhesus
Tissue
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Adipose
Aorta
Artery
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Blood
Blood Vessel
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Bone Marrow
Brain
Breast
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Cerebral Cortex
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Cornea
Coronary Artery
Dermis
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Embryo
Endometrium
Epidermis
Eye
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Hair
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In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
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Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
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Rectum
Retina
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Spinal Cord
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Tail
Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
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Glial Cell
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Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
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HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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