Vectashield Mounting Medium Containing DAPI Works Well

Results Summary

Cells cultured on sterile glass cover slips in 24-well plates were fixed with 4% paraformaldehyde (PFA) in PBS, washed and permeabilized with 0.1% Triton X-100 PBS. The cells were blocked with 1% goat serum for 1 hour and incubated with primary antibody overnight. After washing, the cells were incubated with secondary antibodies for 45 min. Then, the cover slips were mounted with Vectashield mounting medium containing DAPI (Vector Laboratories, Burlingame, USA). The immunostaining images were captured under a Leica fluorescent microscope (DM6000B, Leica, Germany). The results showed that Foxp1ECKO mutants exhibit greater AngII-induced cardiac fibrosis by increased numbers of PCNA/Vimentin (Vim) double positive cells by co-immunostaining in the heart compared with wild-type littermates.