Thermo Fisher Scientific
TrypLE Express
12604013
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Trypsin is a common reagent for detaching adherent cells, but must be stored frozen and activity can decline over time. The TrypLE Express solution acts in a similar way to left adherent cells off of cell culture flasks but can be stored at room temperature and is more stable over time.
Cell culture
Adherent cell lines
May act slower than trypsin for some cell lines.
I add the same volume of TrypLE as I do for trypsin and incubate at 37C, but sometimes longer incubation is needed for cells to fully detach. there is no need to make aliquots for freezing as the whole bottle can be store at room temp with no loss of activity.
N/A
Increased temperature stability.
Somewhat slower than trypsin.
This is a great alternative to trypsin not only for convenience, but is also more gentle on the cells which may shorten their recovery time.