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Brilliant Violet 711 anti-mouse I-A/I-E Antibody
107643
M5/114.15.2
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I-A/I-E (MHC-II) expression in a culture of mixed mouse leukocytes was measured after treating with IFN-gamma for 24hrs.
Flow Cytometry
Primary mouse splenic leukocytes
1:50, 30 minutes on ice
TruStain FcX™ (anti-mouse CD16/32) Antibody
N/A
Cytoflex S flow cytometer (Beckman Coulter)
Increased I-A/I-E MHC-II alloantigens on CD45+ cells following treatment with IFN-gamma was easily measured by flow cytometry with this antibody.
Clone come in many conjugated fluorophores.
High concentration needed for adequate staining.
This antibody worked very well to stain for MHC-II on mouse leukocytes and we will continue to use it in our experiments.