Great Anti-Histone H3 Antibody, Strong Signal

February 27, 2023

Babraham Institute

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (ab1791)

Catalog Number:

ab1791

Clone Number:

N/A

Great antibody for western blotting. Would recommend diluting slightly (1:2000-5000) as very strong signal.

Experimental Design and Results Summary

Applications

Western Blot

Sample

MCF10a cells

Primary Incubation

1:3000 in 5% BSA overnight at 4 degrees

Blocking Agent

5% BSA

Secondary Incubation

1:1000 in 5% BSA for 1 hour at room temperature

Tertiary Incubation

N/A

Detection

ECL reagent

Results Summary

Very strong antibody, nice clean bands with no background noise. Used to compare with acetylated histone 3.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Strong signal.

The Bad

Quite expensive but can reuse.

The Bottom Line

Definitely buy again.

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