Thermo Fisher Scientific
anti-zDHHC15 antibody
PA5-51975
N/A
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This antibody was used to stain zDHHC15 in cultured hippocampal neurons. The antibody is specific and shows significant decrease in staining when validated with shRNA.
Immunofluorescence
Hippocampal neuron culture at DIV 14
1:100, overnight at 4 degrees
5% NDS and 0.3 TritonX 100 in PBS
1:500, 2 hours at RT
None
Antibody specifically defines the localisation of zDHHC15 protein, which is mainly in the golgi compartment. the antibody is specific and a reduction in the immunosignal is seen when a shRNA against zDHCH15 is used.
Specific antibody
Expensive but great.