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APC/Cyanine7 anti-mouse CD19 Antibody
115530
6D5
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease, in this experiment we are looking into B cells (CD19+).
Flow Cytometry
Mouse splenocytes
Surface staining with CD19 APC-Cy7 (1/200), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
In this work, we were looking for surface markers that are differentially expressed by B cells (CD19+) and plasma cells (PC - CD138+).We use CD19 to stain B cells, inside single cells and lymphocytes. As it can be observed, we have the majority of the lymphocytes being B cells in the spleen, as expected.
30005826
Reliable
The signal could be better.
I recommend it, but there are now better options in the market.