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Date Published
Ubiquitin Antibody Did Not Work for Me
(1)
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Novus Biologicals, a Bio-Techne Brand
February 06, 2023
I tried 4 different Ub antibodies (two of which have been published on in my field and protein of interest) and this is one that did not ...
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Good Anti-Mouse CD3 APC-Cy7
(1)
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BioLegend
February 06, 2023
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against ...
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Good Stem Cell Detachment Solution
(1)
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February 06, 2023
We use this accutase solution for routine stem cell detachment. It includes 0.5 mM EDTA•4Na and detaches cell really quickly. It does not ...
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Anti-PE-Cy7-CD206 Antibody Works Well
(1)
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BioLegend
February 06, 2023
One day after ischemia-reperfusion (IR), hearts were perfused with pre-cold PBS. Left ventricle tissues subjected to IR injury were removed ...
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Time Course Effect of Chemoattractant on Rock2 Expression
(1)
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Thermo Fisher Scientific
February 06, 2023
ROCK2 is one of the member of the AGC family of serine/threonine kinases. It it is a regularot for actin-mediated cytoskeleton contractility...
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Excellent Alternative Cell Freezing Solution
(1)
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MilliporeSigma
February 03, 2023
Anyone who performs cell culture knows the importance of having a reliable way to freeze down cells. the standard solution of 10% DMSO in ...
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Complete Electrophoresis System
(1)
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February 03, 2023
We perform a lot of molecular biology (cloning) and mouse colonies genotyping. This little but complete system is very easy to use and ...
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Great Antibody to Detect TIM-3 on T Cells
(1)
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BioLegend
February 03, 2023
I used this antibody to analyze T cells in a panel also analyzing expression of multiple co-inhibitory receptors in mice infected with ...
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Good Antibody to Detect TIGIT on T Cells
(1)
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BioLegend
February 03, 2023
I wanted to study T cell exhaustion/ regulation in mice during the infection with Plasmodium berghei ANKA, a murine model for malaria. TIGIT...
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Easy to Use MCP-1 ELISA Kit
(1)
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R&D Systems, a Bio-Techne Brand
February 03, 2023
I work with infectious bacteria and the goal of this research is to determine the levels of chemokine secretion during in vitro infection. ...
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Good Cryopreservation Medium
(1)
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February 03, 2023
CryoStor CS10 is very good stem cell cryopreservation medium. It maintains majority of stem cell survived and you do not need to use ROCK ...
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Excellent Kit for Direct IgG2a Coupling
(1)
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February 02, 2023
Working on cell surface proteins that are very instable and fast internalized, we needed a fast and reliable kit to directly couple mouse ...
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Bright and Reliable Antibody from BioLegend
(1)
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BioLegend
February 02, 2023
The antibody was extracellularly used on human PBMCs. It has a good quality signal, and it is able to properly separate the positive and ...
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Using MagicMark™ XP Western Protein Standard with a Colometric Ladder is Next Level
(1)
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February 02, 2023
When I started in my grad school lab, I was told that the previous graduate student mixed this ladder with a colorimetric ladder. This ...
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Go-To RNeasy Plus Mini Kit for Years
(1)
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February 02, 2023
We have relied on this specific kit to isolate high quality RNA from thousands, yes thousands, of subjects. Typically ships fast and is ...
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Great PE/Cyanine5 anti-human CD86 Antibody from BioLegend
(1)
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BioLegend
February 02, 2023
The antibody works perfectly on PBMCs. Bright signal and good separation between positive and negative populations.
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Precision Plus Protein™ Standards Work Fine, but Not My Favorite
(1)
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Bio-Rad
February 02, 2023
This is a solid protein ladder to use for western blotting. I personally prefer the Kalediscope because of the variety of colors. With that,...
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Great CD5 Antibody for Mouse Work
(1)
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February 01, 2023
This antibody was used to ascertain the tumour burden of the TCL1 lymphoma in vivo model. This antibody was particularly good at ...
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HA Tag Antibody Did Not Work For Me
(1)
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Thermo Fisher Scientific
February 01, 2023
I tried to use this antibody to verify the coIP of my HA-tagged protein. I saw 'bands' but not at my protein molecular weight an nothing in ...
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Easy, Accurate, and Repeatable Blood & Cell Culture DNA Mini Kit
(1)
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February 01, 2023
As with all QIAGEN kits, this one was extremely user friendly. The included SOP is detailed and thorough. DNA recovery and purity with this ...
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Reliable and Easy Plasmid Purification Kit
(1)
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February 01, 2023
This midi kit is recommended by multiple colleagues, and is easy to perform and reliable. Remember to use the vacuum system and save lots of...
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Thermo Fisher's iBlot™ 2 Gel Transfer Device is Easy to Work With, but Small Technical Issues
(1)
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February 01, 2023
The dry transfer system had a big advantage and only took ~7 minutes of waiting. However, the company really needs to optimize the ...
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Excellent LUNA-FX7™ Automated Cell Counter with Great Customer Service
(1)
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Logos Biosystems
February 01, 2023
After reviewing about 10+ automated cell counters, I finally decided on the Luna-FX7 and do not regret it. A sales-rep reached out to me and...
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Sonic Dismembrator Does its Job
(1)
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Fisher Scientific
January 31, 2023
We've used this to homogenize peptide formulations with very thick and difficult to mix adjuvants. It has performed exceptionally well. We ...
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FAK Antibody Works Well for Western Blot
(1)
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Cell Signaling Technology
January 31, 2023
I was interested to evaluate cell adhesion and migration in epithelial cells after inducing the cells for 48 hours with PDGF. The antibody ...
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Smad3 (C67H9) Rabbit mAb for Western Blot
(1)
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Cell Signaling Technology
January 31, 2023
I used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 ...
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My Favorite Ladder
(1)
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Bio-Rad
January 31, 2023
This is my favorite protein ladder for western blotting. I think that all other ladders are inferior. This ladder has a different color for ...
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Pierce™ Anti-HA Magnetic Beads are Not Bad, But Not My Favorite
(1)
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Thermo Fisher Scientific
January 31, 2023
My goal was to perform coIP with an HA-tagged version of my protein. I was unable to use typical coIP without the addition of an HA tag ...
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QIAprep Spin Miniprep Kit Works Perfectly
(1)
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January 31, 2023
Used to purify and isolate plasmids from a culture suspension, this kit worked great. Running 12 samples, it took less than 40 minutes to ...
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Great to Identify Exogenous Tf
(1)
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MilliporeSigma
January 30, 2023
I used this to identify exogenous Tf in a solution. Importantly, this is holo-Tf (iron bound). Since I study differences between apo- and ...
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Great His Tag Antibody for Western Blot
(1)
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Abcam
January 30, 2023
Used to determine the efficiency of His-tag protein transfection. It gives multiple bands, but there are other proteins with his tags. Used ...
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RIPA is an Efficient and Ready to Use Cell Lysis Buffer
(1)
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MilliporeSigma
January 30, 2023
We use RIPA Buffer as a reagent for cell lysis when preparing cells lysate for protein quantification. This product is very useful as is ...
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Good Nuclear Control Antibody
(1)
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Proteintech Group Inc
January 30, 2023
Lamin A/C antibody was used as a nuclear protein control. Results in HKE293 cells showed that Lamin A/C was only detected in nuclear sample...
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RAPIDstain Works Better than Coomassie
(1)
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G Biosciences
January 30, 2023
This is a great protein stain! It is more sensitive than Coomassie (the standard) and doesn't take that much more time. I used per the ...
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Great Thy1.2(CD90.2) Antibody for Flow Cytometry
(1)
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BD Biosciences
January 30, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Best Universal Blocking Buffer for Western Blot
(1)
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Bio-Rad
January 27, 2023
This is the best blocking buffer that I have had the pleasure of using for my western blots. I first started with 5% milk in TBST, but ...
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Bioactive Hepcidin Peptide from Peptide Institute
(1)
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Peptide Institute Inc.
January 27, 2023
Hepcidin peptides can be tricky because not all are bioactive. This one is. We confirmed this by demonstrating that hepcidin reduces ...
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Always Reliable, Easy to Perform on MinElute PCR Purification Kit
(1)
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January 27, 2023
This kit allows purification of up to 5 µg PCR products or DNA in low elution (from 10 to 200ul) volumes with or without vacuum. Please ...
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Say Goodbye to Sore Thumbs with Thermo Fisher's Wellwash Microplate Washer
(1)
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Thermo Fisher Scientific
January 27, 2023
Our lab purchased this well washer because of a high workload of plate based assays to be run (ELISA, ELISPOT). Set up was fairly ...
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Good SAPK/JNK Antibody for Western Blot
(1)
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Cell Signaling Technology
January 27, 2023
The antibody was diluted with TBST 1:1000 (v/v) and the membrane was incubated overnight at 4 °C. Fibroblast (MRC-5, human cells line) were ...
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Great Thy1.1 Antibody for Flow Cytometry
(1)
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BioLegend
January 27, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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ProteinTech's Ubiquitin Antibody Is My New Go-To
(1)
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Proteintech Group Inc
January 27, 2023
ProteinTech is getting a line in the acknowledgments of my dissertation for this antibody. I tried 4 different Ub antibodies (two of which ...
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Very Good GL7 PB from BioLegend
(1)
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BioLegend
January 26, 2023
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against ...
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Sufficient Recombinant Hephaestin for Peptide Needs
(1)
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MyBioSource.com
January 26, 2023
I used this peptide to examine a novel protein-protein interaction in a cell-free system. Given that hephaestin is a transmembrane protein, ...
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ZO-1 Antibody from Thermo Fisher Works Well
(1)
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Thermo Fisher Scientific
January 26, 2023
I used this to measure ZO-1 levels in iPSC-derived endothelial cells. The antibody worked fine, but we didn't see a difference of note.
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Nutrient-Rich Microbial Broth
(1)
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Corning Life Sciences
January 26, 2023
In our laboratory we use the Miller's LB microbial broth for maintenance and propagation of Escherichia coli (E.Coli). It works with DNA ...
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Sensitive Substrate Saves Time
(1)
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Thermo Fisher Scientific
January 26, 2023
The SuperSignal™ West Dura Extended Duration Substrate is a very sensitive Chemiluminescence Substrate for detection of HRP-coupled ...
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High-Precision Determination of Protein Concentration
(1)
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Thermo Fisher Scientific
January 26, 2023
In our laboratory we use the Pierce BCA Protein Assay Kit for the determination of the protein concentration in the detection range of 20-2...
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Good CXCR3 Antibody for Immunofluorescence
(1)
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Novus Biologicals, a Bio-Techne Brand
January 25, 2023
The role of endothelial CXCR3 in neuroinflammation was examined with CXCR3 KO mice. The expression of CXCR3 on brain endothelial cells from ...
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Reagent Works Great for Transfections
(1)
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January 25, 2023
I used this to transfect my cells with a custom plasmid to produce an HA-tagged version of a protein. The reagents worked great. I was able ...
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Animal Handling Equipment
Antibodies
Balance / Scale
Beads
Biomolecules
Blotting Equipment
Blotting Membranes and Papers
Camera
Cell Analysis Equipment
Cell Sorting Equipment
Cells and Strains
Centrifuge
Chemicals and Reagents
Chromatography Equipment
Columns
Concentrator
Cooler
Counter
Cytometer
Electrophoresis Equipment
Electroporation Equipment
ELISA Kits
Enzymes
Fluorescence Spectrophotometer
Freezer
Homogenizer
Imaging Equipment
Incubator
Injector
Kits
Labels / Dyes / Stains
Ladders and Markers
Light Sources
Liquid Handling Equipment
Luminometer
Magnetic Beads
Magnets
Mass Spectrometer
Master Mixes
Media
Meter
Microplate Washer
Microscopes
Microtome
Mixer
PCR Equipment
Pipettor
Plate Reader
Precast Gels
Primary Cells
Proteins
Purification Equipment
Refrigerator / Freezer
Rocker
Sample Prep Equipment
Scanners
Services
Shaker
Slides
Software
Sonicator
Spectrometer
Sterilizer
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Substrates
Transfection Equipment
Vacuum Manifolds
Vortexer
Water Bath
Applications
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Western Blot
Flow Cytometry
ELISA
Immunofluorescence
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Block/Neutralize/Inhibit
Cell Isolation
Dot Blot
ChIP
Purification
Staining
Cell Culture
DNA Purification
PCR
RNA Analysis
RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
Electrophoresis
RT-qPCR
Cell Counting
Protein Purification
Next Gen Sequencing (NGS)
Library Construction
Genotyping
Gel Imaging
Protein Assay
Reporter Assay
Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
Chicken
Bovine
Canine
Porcine
Horse
Monkey
C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
Squirrel
Fish
Virus
Other Invertebrate
Xenopus/Amphibian
Drosophila
Goat
Rhesus
Tissue
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Adipose
Aorta
Artery
Bladder
Blood
Blood Vessel
Bone
Bone Marrow
Brain
Breast
Bronchus
Cerebral Cortex
Colon
Cornea
Coronary Artery
Dermis
Duodenum
Ear
Embryo
Endometrium
Epidermis
Eye
Foreskin
Gingiva
Hair
Heart
Hippocampus
Hypothalamus
In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
Lymph Node
Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
Prostate
Rectum
Retina
Skeletal Muscle
Skin
Small Intestine
Spinal Cord
Spleen
Stomach
Striatum
Synovium
Tail
Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
Fibroblast
Glial Cell
Granulocyte
Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
Caco-2
HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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