GAD655 Immunofluorescence-Cultured Neuron

February 28, 2023

BlueskyReddit
Vascular Dysfunction
University of Heidelberg
Postdoctoral Researcher

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Company:

Synaptic Systems

Product Name:

anti-GAD65 antibody

Catalog Number:

#198104

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: GAD65 Antibodies

This antibody was used to label the proportion of inhibitory neurons from hippocampal neuronal cultures.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Hippocampal neuron culture at DIV 14

Primary Incubation

1:500, overnight at 4 degrees

Blocking Agent

5% NDS and 0.3 TritonX 100 in PBS

Secondary Incubation

1:500, 2 hours at RT

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

This product allowed us to find out the proportion of inhibitory neurons in an in vitro culture setup derived from rat hippocampus.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specific antibody.

The Bad

High background

The Bottom Line

Antibody has a great signal, recommended.

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