Easy to Use and High Purity T Cell Isolation Kit

UCSF
BMS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

TCRγ/δ+ T Cell Isolation Kit, mouse

Catalog Number:

130-092-125

The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the immune system– the mammalian defense to homeostatic alteration. Ketogenic diets (KDs) are extremely high in fat and very low in carbohydrates and have been shown to dramatically rewire whole-body metabolism. The KD-induced metabolic adaptation improves many physiological processes including aging, fatty acid utilization, and glucose homeostasis. However, the effects of KDs on host immunity remain less explored1–3. Thus, the overall objective of my PhD studies will be to elucidate how KDs affect the immune response in adaptive and maladaptive contexts.

Experimental Design and Results Summary

Application

Cell isolation

Starting Material

Mouse spleen

Tips

Negative selection kit.

Results Summary

The kit yielded high purity of gamme delta T cell from spleen, which can be used for downstream in vitro culture or FACS analysis.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Reliable results.

The Bad

N/A

The Bottom Line

Very convenient, highly recommended.

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