Anti-GAPDH Antibody from CST Works Well

February 23, 2023

BlueskyReddit
pathology
University of Pittsburgh
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Anti-GAPDH antibody

Catalog Number:

5174

Clone Number:

N/A

Info:Supplier Page View Product Specs

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Foxp1(Forkhead box transcription factor P1)ECKO mutants exhibit greater AngII-induced cardiac fibrosis by elevated expression of α-SMA and collagen type I (Col I), III (Col III) by western blot in the heart compared with wild-type littermates. The results showed that endothelial Foxp1 deletion promotes AngII-induced cardiac fibrosis through increased myofibroblast formation by stimulating fibroblast proliferation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse heart

Primary Incubation

1/1000 dilution 4°C overnight incubation

Blocking Agent

5% milk

Secondary Incubation

1/2000 dilution room temperature 1 h incubation

Tertiary Incubation

Room temperature 10 min incubation

Detection

ECL

Results Summary

Foxp1(Forkhead box transcription factor P1)ECKO mutants exhibit greater AngII-induced cardiac fibrosis by elevated expression of α-SMA and collagen type I (Col I), III (Col III) by western blot in the heart compared with wild-type littermates. The results showed that endothelial Foxp1 deletion promotes AngII-induced cardiac fibrosis through increased myofibroblast formation by stimulating fibroblast proliferation.

DOI or PMID #

31177814

Additional Notes

Figure 2C

Image Gallery

Summary

The Good

The antibody works well as expected.

The Bad

N/A

The Bottom Line

The antibody works well.

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