R&D Systems
Mouse Podocalyxin Antibody
AF1556
N/A
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Podocalyxin was detected in formalin fixed paraffin embedded sections of mouse brain using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at a dilution of 1:500 overnight at 4 °C. DAPI and Hoechst 33342 were used together for nuclear stain. The podocalyxin staining denoted micro vessels in brain sections for barrier disruption analysis by probing for serum protein deposition proximal to brain microvasculature. This image was taken on a Zeiss LSM 710 Confocal Microscope (META) with Zeiss Plan Apo 63x/1.40 oil
Immunohistochemistry
Mouse, brain endothelial cells
1:500, Over-night, 4°C
10% BSA/PBST
Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) (ab150131), 1:500, 150 minutes, Room-Temperature
Confocal Microscopy
Brain microvascular marker for analysis of serum protein deposition proximal to vasculature as a consequence of a dysfunctional blood-brain barrier.
Reliable and highly specified to endothelial cells.
Expensive
Antibody has great signal. Recommended.