Time Course Effect of Chemoattractant on CD31 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-CD31 antibody

Catalog Number:

ab28364

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: CD31 Antibodies

PECAM-1 otherwise called CD31is a protein that in humans is encoded by the PECAM1. It is one of the highly glycosylated protein with size of 130 kDa. PECAM-1 plays a key role in removing aged neutrophils from the body. Here we checked the time course effect of chemoattractant on CD31 expression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 prepared in 1x TBST

Blocking Agent

5% Skim milk

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We performed western blot and detect, analyzed the CD31 expression. We found that there is a a time dependent increase of CD31 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This is a antibody and works great for western blot.

The Bad

None

The Bottom Line

Works great for western blot and microscope. Highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion