Time Course Effect of Chemoattractant on Wnt9b Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

R&D Systems

Product Name:

Mouse Wnt-9b Antibody

Catalog Number:

AF3669

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: Wnt9b Antibodies

Wnt family of genes involved in the production of glycolipoproteins essential for cellular signaling and developmental processes. Wnt9b mainly regulate the canonical Wnt/β-catenin signaling pathway. Wnt9b is important in the function and establishment of the kidneys, because Wnt9 is critical for morphogenesis of the nephron. Here we checked the time course effect of Wnt9b expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 prepared in 1x TBST

Blocking Agent

5% Skim milk

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We performed western blot and detect, analyzed the Wnt9b expression. We found that there is a a time dependent increase of Wnt9b expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This is a antibody and works great for western blot.

The Bad

None

The Bottom Line

It works great for western blot and the signal is very strong. This antibody doesn't give any dark background. Highly recommended.

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