Excellent Giantin Antibody for Immunofluorescence-Cultured Neuron

Vascular Dysfunction
University of Heidelberg
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Synaptic Systems

Product Name:

anti-Giantin antibody

Catalog Number:

#263004

Clone Number:

N/A

Info:Supplier Page View Product Specs

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Neurons from in vitro culture were stained with Giantin antibody to label the golgi apparatus of the cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Hippocampal neuron culture at DIV 14

Primary Incubation

1:500, overnight at 4 degrees

Blocking Agent

10% NDS and 0.3 TritonX 100 in PBS

Secondary Incubation

1:500, 2 hours at RT

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

The antibody allowed us to identify the proper location of golgi apparatus and extended golgi regions in the cell soma and dendrites of neurons in an invitro culture system.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specific antibody

The Bad

None

The Bottom Line

Antibody has a great signal, highly recommended

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