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PE/Cyanine7 anti-mouse CD19 Antibody
115520
6D5
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease, in this experiment we are titrating an anti-CD19, a pan B cell marker.
Flow Cytometry
WT mouse spleen
Surface staining with CD19 PE-Cy7, 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
As it can be observed in the figure, we gate lymphocytes, then singlets. Inside the singlets gate we clearly stain B cells using CD19. This antibody works at a 1/1600 dilution.
Reliable and good signal.
The antibody has great signal at a low dilution.