Abcam
Anti-ENO1 antibody [EPR10863(B)]
ab155102
N/A
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To measure ENO1 levels in a mouse cell line treated with a growth factor, mouse cells at the log phase were treated with the growth, and cells were lysed in RIPA buffer. Proteins were extracted, estimated, and resolved in 10% SDS-PAGE. The resolved proteins were then western transferred in a nitrocellulose membrane and probed with ENO1 antibody. The selection of the ENO1 antibody from Abcam was based on our previous experience with the same antibody.
Western blot
Mouse cell line whole-cell protein lysate
Dilution (1:1000) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degrees celsius.
5% skimmed milk made in 1x TBST.
Goat anti-Rabbit IgG HRP Ab, dilution (1:4000) in 5% skimmed milk made in 1x TBST, 1hr at RT.
None
Enhanced chemiluminescent detection in X-Ray films.
A bright and specific band of ENO1 was detected by the Abcam antibody. However, a few more non-specific proteins band were observed. No effect of treatment on study state levels of ENO1 was also found.
Detected particular band of ENO1.
Other nonspecific bands were detected.
Great antibody.