Millipore Sigma
Cephalexin hydrate
C4895
Info: Supplier Page
I identified a putative cell wall hydrolase through a genetic screen. After creating a knockout of this gene, I aimed to determine its impact on cell wall synthesis as a pilot experiment. To achieve this, I assessed its antibiotic sensitivity using different cell wall-targeting antibiotics, including cephalexin purchased from Sigma.
To assess the antibiotic sensitivity of a cell wall hydrolase knockout..
N/A
None.
A moderate increase in cephalexin sensitivity was observed in the cell wall hydrolase knockout (ΔmepK) when compared to the wild-type (WT) strain, as shown in the figure. However, the antibiotic sensitivity of the ΔmepK mutant was lower when compared to another major cell wall hydrolase mutant (ΔmepS). Nevertheless, the sensitivity increased in the ΔmepS ΔmepK double mutant, as depicted in the figure.
30940749
High quality and reliable product.
Cephalexin offers a great opportunity to understand bacterial cell wall biology. Highly recommended.